| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roth, Abigail M (11)
|
NIHHSC |
30.43 |
29.80 |
-0.63 |
| 2 |
Dahl, Sydney R (12)
|
NIPVSC |
32.27 |
31.82 |
-0.45 |
| 3 |
Maybee, Morgan L (12)
|
NISAAC |
32.97 |
32.75 |
-0.22 |
| 4 |
Fosberg, Ella G (11)
|
NISTAR |
34.88 |
34.63 |
-0.25 |
| 5 |
Merrill, Cameron R (12)
|
NIGWST |
38.27 |
39.31 |
1.04 |
| 6 |
Doxey, Ashley A (12)
|
NIEMAC |
44.09 |
40.72 |
-3.37 |
| 7 |
Hammond, Taylor M (12)
|
NIEMAC |
49.83 |
41.00 |
-8.83 |
| 8 |
Fisher, Madalyn J (12)
|
NIPVSC |
50.27 |
46.60 |
-3.67 |
| 9 |
Colburn, Lucy C (11)
|
NISTAR |
|
47.15 |
- |
| 10 |
Wetherby, Breanne A (12)
|
NIEMAC |
55.57 |
52.17 |
-3.40 |
|
Whitepigeon, Breana M (11)
|
NIGWST |
42.00 |
NS |
- |
|
WhitePigeon, Keely R (12)
|
NIGWST |
36.29 |
NS |
- |
|
Denham, Myanna M (11)
|
NISTAR |
37.51 |
NS |
- |
|
Pierino, Kelly E (11)
|
NISTAR |
39.89 |
NS |
- |
|
Reimann, Meghan R (11)
|
NISTAR |
33.86 |
NS |
- |