| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gowetski, JJ (12)
|
MYST |
27.23 |
27.15 |
-0.08 |
| 2 |
Bloss, Maura (12)
|
MYST |
27.19 |
27.19 |
0.00 |
| 3 |
Chase, Bella (11)
|
COR |
27.82 |
28.15 |
0.33 |
| 4 |
Spence, Mackenzie (11)
|
COR |
28.69 |
28.65 |
-0.04 |
| 5 |
Russell, Emily (12)
|
OCY |
28.03 |
28.78 |
0.75 |
| 6 |
Vogt, Rylee (11)
|
ZZESYS |
29.53 |
29.86 |
0.33 |
| 7 |
Austin, Havana (12)
|
COUN |
29.96 |
29.93 |
-0.03 |
| 8 |
Cheney, Brielle (11)
|
MYST |
29.92 |
29.99 |
0.07 |
| 9 |
Johnson, Kirsten (12)
|
WRN |
30.59 |
30.08 |
-0.51 |
| 10 |
Whitlow, Mallory J (12)
|
NIBRAD |
28.32 |
30.11 |
1.79 |
| 11 |
Freer, Grace (12)
|
ZZESYS |
32.88 |
30.44 |
-2.44 |
| 12 |
Wilhelm, Morgan (11)
|
ZZESYS |
30.94 |
30.45 |
-0.49 |
| 13 |
Svolos, Ansley (12)
|
OCY |
30.96 |
30.62 |
-0.34 |
| 14 |
Spitz, Lindsey (12)
|
YJET |
31.01 |
30.89 |
-0.12 |
| 15 |
Bailey, Peytyn (12)
|
MYST |
29.66 |
31.08 |
1.42 |
| 16 |
Gracey-Dalton, Kendall L (11)
|
NIBRAD |
30.92 |
31.17 |
0.25 |
| 17 |
McAvoy, Elsa (12)
|
YJET |
32.09 |
31.73 |
-0.36 |
| 18 |
Smith, Paige (11)
|
NIBRAD |
32.88 |
32.16 |
-0.72 |
| 19 |
Sokolski, Sara (12)
|
WRN |
31.43 |
32.20 |
0.77 |
| 20 |
Lucks, Summer (11)
|
WRN |
31.75 |
32.28 |
0.53 |
| 21 |
Faulk, Morgan (11)
|
YJET |
33.28 |
32.29 |
-0.99 |
| 22 |
Moats, Taylor (11)
|
COR |
31.95 |
32.31 |
0.36 |
| 23 |
Colwell, Sara (11)
|
COR |
32.10 |
32.37 |
0.27 |
| 24 |
Grijalva, Alexis (12)
|
YJET |
32.64 |
32.76 |
0.12 |
| 25 |
Brook, Eliza (11)
|
WRN |
31.44 |
33.23 |
1.79 |
| 26 |
Canfield, Alyson (11)
|
YJET |
35.43 |
34.65 |
-0.78 |
| 27 |
Watkins, Britta (12)
|
YJET |
34.80 |
34.67 |
-0.13 |
| 28 |
Lumia, Elsa (11)
|
YJET |
36.28 |
34.76 |
-1.52 |
| 29 |
Mucha, Olivia (11)
|
TYST |
35.24 |
35.50 |
0.26 |
| 30 |
Burch, Chelsea (11)
|
ZZESYS |
35.63 |
35.53 |
-0.10 |
| 31 |
Johnson, Miranda (11)
|
COR |
36.47 |
35.59 |
-0.88 |
| 32 |
Roach, Hannah (11)
|
COR |
36.76 |
35.79 |
-0.97 |
| 33 |
Campogiani, Adriana (11)
|
NIBRAD |
38.37 |
36.87 |
-1.50 |
| 34 |
Bosco, Jenna (11)
|
MYST |
36.69 |
37.43 |
0.74 |
| 35 |
Dvorak, Evabelle (11)
|
COUN |
38.37 |
37.72 |
-0.65 |
| 36 |
Wilson, Grace (11)
|
COR |
35.66 |
38.20 |
2.54 |
| 37 |
Conroe, Annie (11)
|
YJET |
40.20 |
38.84 |
-1.36 |
| 38 |
Lander, Eden (11)
|
ZZESYS |
40.37 |
39.36 |
-1.01 |
| 39 |
Dixon, Emilee (11)
|
OCY |
39.13 |
39.44 |
0.31 |
| 40 |
Waterman, Renee (12)
|
YJET |
42.75 |
41.97 |
-0.78 |
| 41 |
Demeis, Keely (12)
|
IHYS |
40.34 |
41.99 |
1.65 |
| 42 |
Dalton, Alyson (11)
|
YJET |
46.26 |
42.25 |
-4.01 |
| 43 |
Lindstrom, Kelly (11)
|
YJET |
53.03 |
58.42 |
5.39 |