| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tacik, Morgan E (19)
|
NCTYDE |
27.66 |
28.46 |
0.80 |
| 2 |
Diacumakos, Alexandria R (18)
|
NCRSA |
28.67 |
29.27 |
0.60 |
| 3 |
McGill, Meredith D (17)
|
NCHPSC |
27.96 |
29.94 |
1.98 |
| 4 |
Bowman, Elizabeth (17)
|
NCHPSC |
28.70 |
30.61 |
1.91 |
| 5 |
Hooten, Bailey H (17)
|
NCTYDE |
28.44 |
30.73 |
2.29 |
| 6 |
Van Luvender, Harley E (15)
|
NCRSA |
31.23 |
31.19 |
-0.04 |
| 7 |
Hall, Kristina L (15)
|
NCTYDE |
30.93 |
31.26 |
0.33 |
| 8 |
Blackwell, Madeline C (15)
|
NCHPSC |
30.29 |
31.30 |
1.01 |
| 9 |
McChesney, Karly M (16)
|
NCTYDE |
31.32 |
32.35 |
1.03 |
| 10 |
Milem, Emma G (17)
|
NCRACY |
29.34 |
32.38 |
3.04 |
| 11 |
Filiault, Sophia K (15)
|
NCLOY |
33.48 |
34.55 |
1.07 |
| 12 |
Levin, Lily E (16)
|
NCRSA |
33.00 |
34.91 |
1.91 |
| 13 |
Aldridge, Olivia A (15)
|
NCLOY |
35.23 |
35.51 |
0.28 |
| 14 |
Little, Ashley M (18)
|
NCRSA |
32.92 |
35.64 |
2.72 |
| 15 |
Hall, Sarah E (15)
|
NCHPSC |
46.26 |
36.47 |
-9.79 |
|
Conroy, Erin P (18)
|
NCRSA |
29.86 |
NS |
- |
|
Van Tassel, Lindsey S (17)
|
NCRSA |
30.07 |
NS |
- |