| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thoennes, Emma R (8)
|
MAGSA |
51.25 |
48.08 |
-3.17 |
| 2 |
Tallapragada, Harshitha T (8)
|
MAMSA |
52.17 |
50.05 |
-2.12 |
| 3 |
Ryan, Maxalyn E (8)
|
MADST |
54.54 |
51.19 |
-3.35 |
| 4 |
Kellner, Gabriella H (8)
|
MACBST |
51.43 |
51.79 |
0.36 |
| 5 |
Treglia, Megan E (8)
|
MAJW |
48.73 |
51.85 |
3.12 |
| 6 |
Dennis, Haley R (8)
|
MAJW |
50.73 |
52.66 |
1.93 |
| 7 |
Kraft, Olivia G (8)
|
MADCAA |
54.10 |
53.15 |
-0.95 |
| 8 |
Cohen, Sarah L (8)
|
MAJW |
53.47 |
53.35 |
-0.12 |
| 9 |
Broderick, Ava M (8)
|
MAJW |
54.88 |
54.98 |
0.10 |
| 10 |
Prior, Bethany (8)
|
MAMSA |
|
57.78 |
- |
| 11 |
Campbell, Harbor M (7)
|
MADCAA |
|
58.30 |
- |
| 12 |
Gounder, Kavya R (8)
|
MAGSA |
|
58.67 |
- |
| 13 |
Sheehan, Reagan M (7)
|
MAJW |
58.33 |
59.88 |
1.55 |
| 14 |
Barbich, Eva M (8)
|
MAPRSC |
59.41 |
1:00.07 |
0.66 |
| 15 |
Wang, Mira (8)
|
MAJW |
|
1:02.60 |
- |
| 16 |
Chung, Emma R (7)
|
MAJW |
|
1:25.64 |
- |
|
Albuquerque, Sylvia G (7)
|
MAJW |
|
DQ |
- |
|
Cochran, Lauren E (7)
|
MADST |
|
DQ |
- |
|
Lee, Katherine Y (8)
|
MAJW |
|
NS |
- |
|
Liu, Emma K (7)
|
MAJW |
|
NS |
- |
|
Devers, Abigal B (7)
|
MAJW |
|
NS |
- |
|
Phillips, Alexandra J (8)
|
MADST |
49.71 |
NS |
- |
|
Sommer, Truly (8)
|
MAMAC |
45.93 |
NS |
- |