| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (12)
|
NIVELO |
25.52 |
28.59 |
3.07 |
| 2 |
Chung, Emily (12)
|
NICLSC |
29.67 |
29.93 |
0.26 |
| 3 |
Wamsley, Sarah E (11)
|
NIVELO |
28.23 |
31.53 |
3.30 |
| 4 |
Barros, Rachel (12)
|
NIUN-B |
29.00 |
31.81 |
2.81 |
| 5 |
Anderson, Madden (11)
|
NIVELO |
28.60 |
32.20 |
3.60 |
| 6 |
Glor, Paige R (12)
|
NITTSC |
33.79 |
32.63 |
-1.16 |
| 7 |
Roeser, Lila J (12)
|
NITTSC |
33.87 |
34.22 |
0.35 |
| 8 |
Scibetta, Madeline C (12)
|
NITTSC |
37.36 |
34.26 |
-3.10 |
| 9 |
Lyons, Lucy E (11)
|
NITTSC |
35.16 |
34.60 |
-0.56 |
| 10 |
Barillari, Emma (12)
|
NIVELO |
31.56 |
34.99 |
3.43 |
| 11 |
Gentile, Olivia (12)
|
NIVELO |
32.31 |
35.96 |
3.65 |
| 12 |
Shepherd, Taylor R (11)
|
NITTSC |
36.95 |
36.03 |
-0.92 |
| 13 |
Sperry, Lola E (12)
|
NITTSC |
38.63 |
37.97 |
-0.66 |
| 14 |
Fabiano, Mackenzie G (11)
|
NIHHSC |
35.91 |
39.14 |
3.23 |
| 15 |
Winker, Delilah M (11)
|
NITTSC |
39.29 |
39.59 |
0.30 |
| 16 |
Jones, Evelyn V (12)
|
NIVELO |
39.25 |
40.84 |
1.59 |
| 17 |
Hesch, Addison L (11)
|
NITTSC |
34.21 |
42.23 |
8.02 |
|
McCann, Madaline S (11)
|
NIRAYS |
|
NS |
- |
|
Crozier, Sydney (11)
|
NIVELO |
36.84 |
NS |
- |
|
Hennessey, Caitlin G (11)
|
NIVELO |
45.73 |
NS |
- |
|
Kappel, Alexandra H (11)
|
NIVELO |
43.60 |
NS |
- |