| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stapleton, Lily (9)
|
MAJY |
41.79 |
39.65 |
-2.14 |
| 2 |
Simeone, Samantha M (10)
|
MAJY |
43.17 |
42.40 |
-0.77 |
| 3 |
Kessinger, Gabriella M (10)
|
MAKAY |
|
42.60 |
- |
| 4 |
Cicero, Ryann E (9)
|
MAWCAY |
43.61 |
42.68 |
-0.93 |
| 5 |
Hung, Abigail R (8)
|
MAWCAY |
|
42.77 |
- |
| 6 |
Shuda, Francesca R (9)
|
MARKRY |
51.82 |
43.10 |
-8.72 |
| 7 |
Martelli, Peyton F (10)
|
MARKRY |
43.93 |
43.23 |
-0.70 |
| 8 |
Dunn, Kelly B (9)
|
MAWCAY |
47.58 |
44.29 |
-3.29 |
| 9 |
Persch, Chloe (9)
|
MAKAY |
|
44.70 |
- |
| 10 |
Mangan, Madeline (10)
|
MAJY |
|
45.40 |
- |
| 11 |
Peterson, Avery L (10)
|
MABYNS |
53.66 |
47.07 |
-6.59 |
| 12 |
Inners, Elizabeth (9)
|
MABCY |
52.87 |
47.39 |
-5.48 |
| 13 |
Hayes, Kathryn E (9)
|
MAWCAY |
|
49.75 |
- |
| 14 |
Owens, Katie E (7)
|
MAJY |
50.61 |
51.15 |
0.54 |
| 15 |
Stein, Alexandra M (9)
|
MAWCAY |
46.50 |
53.18 |
6.68 |
| 16 |
Lafferty, Addison G (10)
|
MAKAY |
|
53.80 |
- |
| 17 |
Zou, Kara Q (10)
|
MAKAY |
|
53.84 |
- |
| 18 |
Nelson, Lucia I (10)
|
MAWCAY |
53.47 |
54.92 |
1.45 |
| 19 |
Halloran, Corrine M (10)
|
MAWCAY |
53.89 |
57.18 |
3.29 |
| 20 |
Knecht, Evelyn C (9)
|
MAPAY |
|
57.95 |
- |
| 21 |
Leitgeb, Lacey M (8)
|
MAJY |
|
58.67 |
- |
| 22 |
Krieger, Kelsie (8)
|
MAKAY |
|
59.18 |
- |
| 23 |
Silva-Trego, Mary Jane (9)
|
MABCY |
|
59.51 |
- |
| 24 |
Rose, Olivia A (9)
|
MAWCAY |
1:02.41 |
59.56 |
-2.85 |
| 25 |
Shearer, Mara (8)
|
MAPAY |
|
59.87 |
- |
| 26 |
Viner, Reagan E (9)
|
MARKRY |
1:15.90 |
1:09.77 |
-6.13 |
| 27 |
Gagliardi, Raegan (8)
|
MAWBY |
1:14.07 |
1:19.15 |
5.08 |