| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holian, Tanner J (15)
|
NCUN-G |
22.35 |
22.74 |
0.39 |
| 2 |
Huitsing, Jacob H (16)
|
NCGCY |
23.34 |
23.85 |
0.51 |
| 3 |
Smith, Sawyer J (15)
|
NCGCY |
25.22 |
24.60 |
-0.62 |
| 4 |
McDonald, Benjamin M (18)
|
NCGCY |
23.94 |
24.66 |
0.72 |
| 5 |
Alker, Brandon R (15)
|
NCGCY |
25.27 |
25.22 |
-0.05 |
| 6 |
Houser, Austin J (16)
|
NCTYDE |
25.67 |
25.95 |
0.28 |
| 7 |
Nadolski, Patrick T (17)
|
NCYBAC |
25.42 |
26.31 |
0.89 |
| 8 |
Brouwer, Ethan G (17)
|
NCYBAC |
26.92 |
27.10 |
0.18 |
| 9 |
Nicolet, Foster J (15)
|
NCYBAC |
26.84 |
27.12 |
0.28 |
| 10 |
Ellis, Andrew T (16)
|
NCYBAC |
27.38 |
27.82 |
0.44 |
| 11 |
Clendinen, Nathaniel J (16)
|
NCGCY |
28.24 |
28.09 |
-0.15 |