| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yorkery, Cadence A (9)
|
NCCAT |
41.74 |
43.87 |
2.13 |
| 2 |
Brown, Makena R (9)
|
NCSGSA |
48.83 |
45.80 |
-3.03 |
| 3 |
Wolff, Ameilia N (10)
|
NCSFSC |
50.64 |
46.06 |
-4.58 |
| 4 |
Collins, Taylor E (10)
|
NCHPSC |
47.41 |
46.08 |
-1.33 |
| 5 |
Strasser, Morgan G (9)
|
NCSFSC |
51.37 |
46.51 |
-4.86 |
| 6 |
Wright, Lillian E (9)
|
NCSGSA |
43.85 |
47.58 |
3.73 |
| 7 |
Day, Lauren E (10)
|
NCHPSC |
44.90 |
47.69 |
2.79 |
| 8 |
Isaac, Claire R (9)
|
NCEAC |
56.99 |
48.05 |
-8.94 |
| 9 |
LaRue, Bailey A (9)
|
NCHPSC |
52.99 |
48.92 |
-4.07 |
| 10 |
Howards, Nina P (10)
|
NCEAC |
49.92 |
49.04 |
-0.88 |
| 11 |
O'Sullivan, Morgan S (10)
|
NCEAC |
46.99 |
49.44 |
2.45 |
| 12 |
Cummings, Isa L (9)
|
NCUN |
48.74 |
51.30 |
2.56 |
| 13 |
Vest, Sadie A (9)
|
NCSGSA |
55.37 |
52.02 |
-3.35 |
| 14 |
Williams, Amber R (9)
|
NCCAT |
|
54.26 |
- |
| 15 |
Grove, Kaye T (10)
|
NCSGSA |
47.60 |
56.06 |
8.46 |
| 16 |
Thornton, Braedy A (9)
|
NCSGSA |
1:06.36 |
1:02.66 |
-3.70 |
| 17 |
Reynolds, Lily C (9)
|
NCSFSC |
|
1:05.18 |
- |
| 18 |
Knable, Lilliana M (9)
|
NCHPSC |
1:08.13 |
1:05.61 |
-2.52 |
| 19 |
Weymouth, Gracie D (10)
|
NCEAC |
58.04 |
1:07.95 |
9.91 |
|
Goin, Ella R (10)
|
NCCAT |
56.49 |
NS |
- |
|
Velazquez, Makeala E (10)
|
NCCAT |
54.86 |
NS |
- |