| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bole, Tanvi (10)
|
OHWTRC |
2:38.75 |
2:31.35 |
-7.40 |
| 2 |
Woods, Aubriana A (10)
|
OHDR |
2:36.17 |
2:31.95 |
-4.22 |
| 3 |
Offenburger, Casey J (9)
|
OHMAKO |
2:34.64 |
2:32.04 |
-2.60 |
| 4 |
Nuttbrock, Kennedy L (10)
|
OHDR |
2:40.55 |
2:34.50 |
-6.05 |
| 5 |
Havrilla, Audrey E (9)
|
OHDR |
2:34.08 |
2:36.34 |
2.26 |
| 6 |
Sparks, Madison K (10)
|
OHMAKO |
2:46.09 |
2:43.73 |
-2.36 |
| 7 |
Pasquale, Cailin J (9)
|
OHMAKO |
2:47.47 |
2:45.46 |
-2.01 |
| 8 |
Kaeff, Taylor A (10)
|
OHDR |
2:47.88 |
2:45.61 |
-2.27 |
| 9 |
Pyo, Olivia J (10)
|
OHDR |
2:48.31 |
2:45.85 |
-2.46 |
| 10 |
Dean, Michelle M (9)
|
OHDR |
2:40.91 |
2:48.12 |
7.21 |
| 11 |
Eshman, Bridgette A (9)
|
OHLIFE |
3:00.00 |
2:54.92 |
-5.08 |
| 12 |
Nichols, Aria C (9)
|
OHMAKO |
3:02.67 |
2:56.54 |
-6.13 |
| 13 |
Mitiek, Amena M (10)
|
OHLIFE |
2:50.00 |
2:56.57 |
6.57 |
| 14 |
Stedronsky, Samantha M (10)
|
OHMAC |
3:10.20 |
2:57.33 |
-12.87 |
| 15 |
Howell, Skylar R (9)
|
OHKEY |
3:03.38 |
2:58.10 |
-5.28 |
| 16 |
Muroski, Isla C (9)
|
OHMAC |
3:07.56 |
2:59.00 |
-8.56 |
| 17 |
Spalding, Hadley A (10)
|
OHDR |
3:05.99 |
3:00.41 |
-5.58 |
| 18 |
Smith, Valerie M (9)
|
OHKEY |
3:08.30 |
3:01.29 |
-7.01 |
| 19 |
Kaesemeyer, Taylor A (10)
|
OHLIFE |
3:16.58 |
3:12.90 |
-3.68 |
| 20 |
Ozer, Defne (9)
|
OHLIFE |
2:50.00 |
3:27.50 |
37.50 |
| 21 |
Susag, Anna R (10)
|
OHBAC |
3:36.64 |
3:28.37 |
-8.27 |
| 22 |
Conti, Ava C (10)
|
OHLIFE |
3:27.28 |
3:38.55 |
11.27 |
| 23 |
Golde, Jenna E (8)
|
OHBAC |
4:00.00 |
4:05.34 |
5.34 |
| 24 |
Nancarrow-Ibarra, Aria I (7)
|
OHDR |
4:31.59 |
4:28.35 |
-3.24 |
|
Yang, Allison (10)
|
OHLIFE |
3:10.00 |
NS |
- |
|
Lay, Aliyah K (10)
|
OHKEY |
3:13.83 |
NS |
- |