| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spetz, Marlena A (7)
|
OHLAKY |
21.23 |
22.36 |
1.13 |
| 2 |
Karthikeyan, Harine (7)
|
OHRAYS |
26.12 |
23.09 |
-3.03 |
| 3 |
Kasten, Ella R (7)
|
OHRAYS |
26.35 |
24.82 |
-1.53 |
| 4 |
Nancarrow-Ibarra, Aria I (7)
|
OHDR |
26.88 |
24.88 |
-2.00 |
| 5 |
Banning, Esmae A (7)
|
OHKEY |
26.76 |
25.83 |
-0.93 |
| 6 |
Hall, Charley B (7)
|
OHKEY |
27.21 |
26.73 |
-0.48 |
| 7 |
Haas, Bella E (7)
|
OHKEY |
28.16 |
26.99 |
-1.17 |
| 8 |
Geiman, Johanna D (7)
|
OHKCST |
31.01 |
29.64 |
-1.37 |
| 9 |
Kahle, Piper J (7)
|
OHLIFE |
|
31.14 |
- |
| 10 |
Taylor, Madelyn M (7)
|
OHRAYS |
31.22 |
32.39 |
1.17 |
| 11 |
Paris, Delia R (7)
|
OHWTRC |
37.96 |
35.62 |
-2.34 |
|
Kitchen, Poppy J (7)
|
OHDR |
29.89 |
NS |
- |
|
Von Handorf, Harriet J (7)
|
OHKEY |
30.31 |
NS |
- |