| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wingert, Stephen A (10)
|
MAHAC |
36.12 |
35.78 |
-0.34 |
| 2 |
Zagaceta, Magill M (9)
|
MAHAC |
34.87 |
36.38 |
1.51 |
| 3 |
Verbeek, Stefan L (9)
|
MAHAC |
41.15 |
40.54 |
-0.61 |
| 4 |
Lehan, Sean C (9)
|
MAHAC |
40.69 |
42.46 |
1.77 |
| 5 |
Riley, Aadyn J (9)
|
MADSC |
44.33 |
44.32 |
-0.01 |
| 6 |
Griffith, Charlie E (10)
|
MAHAC |
44.96 |
45.89 |
0.93 |
| 7 |
Chappell, William C (9)
|
MAHAC |
49.60 |
46.23 |
-3.37 |
| 8 |
Serfass, Caleb P (9)
|
MAHAC |
47.95 |
48.19 |
0.24 |
| 9 |
Liu, Issac (10)
|
MADSC |
47.54 |
49.77 |
2.23 |
| 10 |
Nguyen, Kyle A (9)
|
MAHAC |
48.27 |
49.81 |
1.54 |
| 11 |
Norton, Aidan M (9)
|
MAHAC |
|
1:24.31 |
- |
|
Fastrich, Owen S (9)
|
MAHAC |
49.61 |
DQ |
- |
|
Wiggins, Maximilian L (9)
|
MALAC |
|
DQ |
- |