| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thamm, Claudia M (14)
|
MANPAC |
53.13 |
1:03.48 |
10.35 |
| 2 |
Schulz, Parker A (14)
|
MANPAC |
52.84 |
1:03.61 |
10.77 |
| 3 |
Goroshko, Julia (14)
|
MACBST |
55.24 |
1:05.21 |
9.97 |
| 4 |
Boland Bintner, Maureen T (14)
|
MANPAC |
56.28 |
1:10.20 |
13.92 |
| 5 |
Von Schmidt, Kylie (14)
|
MANRG |
1:02.66 |
1:10.52 |
7.86 |
| 6 |
Olszewski, Kayleigh S (14)
|
MAPWAC |
1:00.11 |
1:10.76 |
10.65 |
| 7 |
Muth, Haley P (14)
|
MANPAC |
1:01.54 |
1:11.56 |
10.02 |
| 8 |
Petrovia, Arianna M (14)
|
MANRG |
1:00.59 |
1:13.15 |
12.56 |
| 9 |
Peters, Abigail E (14)
|
MANPAC |
1:01.44 |
1:13.66 |
12.22 |
| 10 |
Lochstet, Claresta E (14)
|
MAUDAC |
1:02.84 |
1:18.62 |
15.78 |
| 11 |
Broadhead, Abigail C (14)
|
MANPAC |
1:07.97 |
1:20.52 |
12.55 |
| 12 |
Rane, Shefalee S (14)
|
MAUN! |
1:08.90 |
1:22.53 |
13.63 |