| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Daly, Kaelan G (14)
|
MANPAC |
53.62 |
1:06.13 |
12.51 |
| 2 |
Mikalic, Maggie (14)
|
MAUDAC |
56.45 |
1:06.80 |
10.35 |
| 3 |
Sapozhnikov, Katherina A (14)
|
MAUDAC |
56.60 |
1:07.03 |
10.43 |
| 4 |
Krause, Laurel E (14)
|
MAPSC |
56.49 |
1:07.14 |
10.65 |
| 5 |
Fritz, Madison L (14)
|
MANPAC |
55.68 |
1:09.33 |
13.65 |
| 6 |
Thamm, Charlotte P (14)
|
MANPAC |
56.52 |
1:11.54 |
15.02 |
| 7 |
Hill, Corinne E (14)
|
MABLUE |
1:00.53 |
1:11.57 |
11.04 |
| 8 |
Rosa, Isabella A (14)
|
MABLUE |
59.22 |
1:11.73 |
12.51 |
| 9 |
Koerper, Elizabeth R (14)
|
MANPAC |
59.57 |
1:12.02 |
12.45 |
| 10 |
Hammond, Kayla A (14)
|
MATRI |
1:00.14 |
1:13.04 |
12.90 |
| 11 |
Link, Natalie C (14)
|
MAPSC |
1:02.99 |
1:14.93 |
11.94 |
| 12 |
Mangino, Lila M (14)
|
MABLUE |
1:05.15 |
1:17.06 |
11.91 |
| 13 |
Winters, Natalie J (14)
|
MABLUE |
1:04.77 |
1:17.64 |
12.87 |
|
Miller, Hannah S (14)
|
MABLUE |
55.89 |
NS |
- |
|
Perez, Brianna (14)
|
MABLUE |
1:01.80 |
NS |
- |