| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hosley, Abrianna (10)
|
MALHY |
41.60 |
40.63 |
-0.97 |
| 2 |
Boomer-Brazier, Maggie (10)
|
MASCAY |
41.83 |
43.32 |
1.49 |
| 3 |
Nestor, Elizabeth (9)
|
MALHY |
46.40 |
44.99 |
-1.41 |
| 4 |
Barshinger, Sydney (10)
|
MASCAY |
44.99 |
46.83 |
1.84 |
| 5 |
Wafula, Edna (10)
|
MASCAY |
45.01 |
47.21 |
2.20 |
| 6 |
Pietraski, Abigail G (10)
|
ELY |
51.32 |
47.25 |
-4.07 |
| 7 |
Zettlemoyer, Addison E (9)
|
ELY |
47.94 |
47.34 |
-0.60 |
| 8 |
Coble, Aubrey (9)
|
MASCAY |
46.57 |
48.14 |
1.57 |
| 9 |
Williams, Lilyana (9)
|
MASCAY |
45.72 |
50.18 |
4.46 |
| 10 |
Campbell, Kaitlyn M (10)
|
MASCAY |
52.40 |
52.19 |
-0.21 |
| 11 |
Christensen, Elle (9)
|
MALHY |
|
52.94 |
- |
| 12 |
Williams, Sarah (9)
|
MASCAY |
51.11 |
55.09 |
3.98 |