| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ma, Sally (13)
|
ILHEAT |
23.81 |
23.90 |
0.09 |
| 2 |
Morris, Katherine L (14)
|
ILBRRY |
23.29 |
23.98 |
0.69 |
| 3 |
Gasperko, Elizabeth M (14)
|
ILBRRY |
25.58 |
24.85 |
-0.73 |
| 4 |
Currier, Ashley C (14)
|
ILJETS |
26.13 |
25.52 |
-0.61 |
| 5 |
Currier, Haley S (14)
|
ILJETS |
26.48 |
25.58 |
-0.90 |
| 5 |
Friedman, Anna (14)
|
ILMYST |
25.51 |
25.58 |
0.07 |
| 7 |
Viele, Olivia G (14)
|
ILSPY |
25.69 |
25.83 |
0.14 |
| 8 |
Rosenthal, Abigail R (14)
|
ILBRRY |
25.84 |
25.84 |
0.00 |
| 9 |
Nickoley, Anna K (13)
|
ILBRRY |
26.23 |
25.85 |
-0.38 |
| 10 |
Muck, Abigail J (14)
|
ILDCST |
26.53 |
25.90 |
-0.63 |
| 11 |
Koltuniuk, Julia (14)
|
ILSAGE |
25.98 |
26.06 |
0.08 |
| 12 |
Heagy, Peyton E (13)
|
ILILVY |
26.02 |
26.18 |
0.16 |
| 13 |
Nietzel, Makayla L (14)
|
ILSAGE |
26.32 |
26.23 |
-0.09 |
| 14 |
Trembly, Kate N (14)
|
ILSAGE |
26.55 |
26.37 |
-0.18 |
| 15 |
Bushroe-Stumpf, Morrigan K (13)
|
ILMYST |
25.87 |
26.39 |
0.52 |
| 16 |
Muck, Dellana J (14)
|
ILDCST |
26.55 |
26.42 |
-0.13 |
| 17 |
Papaleka, Dea I (13)
|
ILLATT |
26.96 |
26.56 |
-0.40 |
| 17 |
Stream, Laura M (14)
|
ISSAMY |
26.70 |
26.56 |
-0.14 |
| 19 |
McKibbon, Cali A (14)
|
ILKEWE |
26.42 |
26.66 |
0.24 |
| 20 |
Skrzypek, Nicole D (13)
|
ILDCST |
26.48 |
26.67 |
0.19 |
| 21 |
Points, Mia K (14)
|
ILDOCS |
27.01 |
26.87 |
-0.14 |
| 22 |
Massmann, Ava L (13)
|
ILTOPS |
27.07 |
26.96 |
-0.11 |
| 23 |
Gawedzinski, Emily A (14)
|
ILSPY |
27.03 |
27.27 |
0.24 |
| 24 |
Antonacci, Teresa M (14)
|
ILSPY |
26.26 |
27.43 |
1.17 |
| 25 |
McMahon, Josephine C (14)
|
ILDCST |
26.62 |
27.52 |
0.90 |
| 26 |
Tate, Cally (13)
|
WCY |
27.04 |
27.66 |
0.62 |
|
Kiley, Bryn E (14)
|
ILRAYS |
25.79 |
DQ |
- |