| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCottry, Kada S (8)
|
MADCAA |
38.86 |
39.18 |
0.32 |
| 2 |
Hahn, Farrah J (8)
|
MAPSC |
41.04 |
40.55 |
-0.49 |
| 3 |
Capriotti, Vita R (7)
|
MAPWAC |
44.08 |
42.59 |
-1.49 |
| 4 |
Zochowski, Alexa E (8)
|
MAUDAC |
|
45.76 |
- |
| 5 |
Fedorenko, Alisa (8)
|
MABSC |
|
51.47 |
- |
| 6 |
Boczkowski, Stella W (8)
|
MASAKA |
51.05 |
51.54 |
0.49 |
| 7 |
Liharik, Hanna E (8)
|
MAPCAC |
50.33 |
51.76 |
1.43 |
| 8 |
Navarro, Addison (8)
|
MABSC |
|
51.93 |
- |
| 9 |
Gandhi, Veda N (8)
|
MAPSC |
|
53.17 |
- |
| 10 |
Simmons, Carly (7)
|
MAT85 |
53.18 |
53.19 |
0.01 |
| 11 |
McGarry, Lucy E (8)
|
MABSC |
|
53.42 |
- |
| 12 |
Renion, Anica J (8)
|
MAPSC |
|
53.92 |
- |
| 13 |
Dojack, Paisley K (8)
|
MAPSC |
51.49 |
54.37 |
2.88 |
| 14 |
Archer, Miracle C (8)
|
MATHY |
52.44 |
56.07 |
3.63 |
| 15 |
Shah, Sayna V (8)
|
MABSC |
|
56.17 |
- |
| 16 |
Schultz, Kenzi M (8)
|
MAPSC |
1:01.36 |
58.57 |
-2.79 |
| 17 |
Ginesin, Celia F (8)
|
MABSC |
|
59.83 |
- |
| 18 |
Smith, Nikoline C (8)
|
MAUDAC |
|
1:04.35 |
- |
|
Boczkowski, Cordelia W (6)
|
MASAKA |
|
DQ |
- |
|
Piccini, Colleen P (8)
|
MAPSC |
1:01.69 |
DQ |
- |
|
Lightman, Chloe A (8)
|
MABSC |
|
DQ |
- |
|
Molnarko, Emma G (8)
|
MAPSC |
1:02.28 |
NS |
- |