| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nicholls, Brant C (10)
|
MAJW |
49.53 |
42.20 |
-7.33 |
| 2 |
Baldino, Zac P (10)
|
MASSC |
49.19 |
44.81 |
-4.38 |
| 3 |
Mann, Taylor J (10)
|
MABLUE |
50.24 |
46.70 |
-3.54 |
| 4 |
Shevade, Dhruv J (10)
|
MAPWAC |
53.54 |
48.68 |
-4.86 |
| 5 |
Sommerkamp, Jayson E (10)
|
MATWAC |
51.03 |
49.48 |
-1.55 |
| 6 |
Suren, Anirudh (10)
|
MAT85 |
50.16 |
49.65 |
-0.51 |
| 7 |
Jawork, Jack (10)
|
MAFSA |
49.87 |
51.06 |
1.19 |
| 8 |
Sao, Shreyas (10)
|
MAMSA |
53.00 |
51.40 |
-1.60 |
| 9 |
Porter, Brendan M (10)
|
MAWA |
48.25 |
51.66 |
3.41 |
| 10 |
Cratin, Ace J (10)
|
MAGAAC |
52.08 |
52.80 |
0.72 |
| 11 |
Schneider, Tristan C (10)
|
MAJW |
54.84 |
53.66 |
-1.18 |
| 12 |
Hoang, Thomas N (10)
|
MAPAAC |
56.77 |
58.71 |
1.94 |
| 13 |
Loh, Jason K (10)
|
MAGSA |
1:02.27 |
59.76 |
-2.51 |
|
Coyle, Liam P (10)
|
MATRI |
1:33.55 |
DQ |
- |
|
Fanini, Rocco M (10)
|
MAGPAC |
57.18 |
NS |
- |