| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Worthen, Jermaine D (15)
|
PVPGPR |
51.35 |
51.78 |
0.43 |
| 2 |
Williams, Andrew J (17)
|
PVRY |
52.66 |
51.90 |
-0.76 |
| 3 |
Orrell, Evan A (17)
|
PVPAC |
52.63 |
53.33 |
0.70 |
| 4 |
Reeves, Kazuki E (14)
|
PVPAC |
53.26 |
53.39 |
0.13 |
| 5 |
Butler, Daniel W (17)
|
PVPGPR |
54.06 |
54.40 |
0.34 |
| 6 |
Khandwalla, Mohamedali (17)
|
PVUN |
55.78 |
55.62 |
-0.16 |
| 7 |
Brandes, Adrian (16)
|
PVUN |
59.66 |
59.68 |
0.02 |
| 8 |
Songu-Mbriwa, Nathanael K (16)
|
PVPGPR |
59.45 |
1:00.36 |
0.91 |
| 9 |
Kim, Daniel D (13)
|
PVTRA |
1:01.17 |
1:00.67 |
-0.50 |
| 10 |
Wang, Nathan N (13)
|
PVRY |
1:08.15 |
1:04.99 |
-3.16 |
| 11 |
Rhoades, Cooper H (15)
|
PVPAC |
1:05.96 |
1:07.21 |
1.25 |
| 12 |
Tiso, Daniel (13)
|
PVUN |
1:10.96 |
1:08.60 |
-2.36 |
| 13 |
Murphy, Alexander (15)
|
PVRY |
1:15.35 |
1:15.07 |
-0.28 |
| 14 |
Dethridge, Alexander A (14)
|
PVPAC |
1:21.05 |
1:17.23 |
-3.82 |
| 15 |
Silver, Elan R (13)
|
PVPAC |
1:24.60 |
1:18.62 |
-5.98 |
| 16 |
Siddiqui, Abraham (14)
|
PVYSS |
|
1:20.77 |
- |