| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xiao, Victoria (14)
|
PVDRAG |
28.40 |
27.37 |
-1.03 |
| 2 |
Cebrian, Francesca A (14)
|
PVDRAG |
27.35 |
27.53 |
0.18 |
| 3 |
Kumar, Neha (14)
|
PVDCPR |
28.74 |
28.70 |
-0.04 |
| 4 |
Goodweather, Isabelle L (14)
|
PVDCPR |
30.38 |
30.00 |
-0.38 |
| 5 |
Chatradi, Sirihamsini (15)
|
PVDRAG |
30.80 |
30.10 |
-0.70 |
| 6 |
Heisler, Alexandra R (14)
|
PVDRAG |
30.98 |
30.73 |
-0.25 |
| 7 |
Sisk, Fabiola D (15)
|
PVDCPR |
35.35 |
32.09 |
-3.26 |
| 8 |
Desel Dolan, Katherine M (14)
|
PVDCPR |
32.82 |
32.94 |
0.12 |
|
Carranza, Isabella S (15)
|
PVDCPR |
28.62 |
NS |
- |
|
Yang, Julia (14)
|
PVMSSC |
31.09 |
NS |
- |