| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lindner, Jacob M (17)
|
PVNCAP |
24.41 |
25.14 |
0.73 |
| 2 |
Mathis, Ryan A (17)
|
PVNCAP |
24.42 |
26.03 |
1.61 |
| 3 |
Vilbig, Umi R (15)
|
PVRMSC |
26.14 |
26.38 |
0.24 |
| 4 |
Kim, Jisu (18)
|
PVRMSC |
25.80 |
26.50 |
0.70 |
| 5 |
Hall, Matthew D (16)
|
PVMSSC |
26.68 |
26.53 |
-0.15 |
| 6 |
Yang, Jing Tang T (15)
|
PVRMSC |
26.39 |
26.54 |
0.15 |
| 7 |
Kim, Minsu (17)
|
PVRMSC |
26.14 |
26.73 |
0.59 |
| 8 |
Bowman, Jack S (17)
|
PVRMSC |
26.77 |
26.94 |
0.17 |
| 9 |
Steinwandel, Matthew D (18)
|
PVRMSC |
26.09 |
27.08 |
0.99 |
| 10 |
Buda, Henry T (18)
|
PVUN-M |
25.62 |
27.11 |
1.49 |
| 10 |
Santorelli, Nicholas J (17)
|
PVRMSC |
26.44 |
27.11 |
0.67 |
| 12 |
Venit III, John (21)
|
PVPAC |
25.92 |
27.16 |
1.24 |
| 13 |
Rooney, Aiden P (16)
|
PVRMSC |
27.37 |
27.19 |
-0.18 |
| 14 |
Wilbur, John C (17)
|
PVRMSC |
26.26 |
27.49 |
1.23 |
| 15 |
Jo, Benjamin W (15)
|
PVRMSC |
27.04 |
27.50 |
0.46 |
| 16 |
Passaro, Thomas J (16)
|
PVRMSC |
28.10 |
27.68 |
-0.42 |
| 17 |
Kendrick, William E (15)
|
PVPAC |
26.97 |
27.73 |
0.76 |
| 17 |
Islam, Azzam S (16)
|
PVRMSC |
27.82 |
27.73 |
-0.09 |
| 19 |
Phan, Ethan D (16)
|
PVRMSC |
28.01 |
27.86 |
-0.15 |
| 20 |
Gao, Vincent R (17)
|
PVTOLL |
27.45 |
27.92 |
0.47 |
| 21 |
Karayianis, Alexander J (15)
|
PVNCAP |
27.12 |
27.95 |
0.83 |
| 22 |
Nguyen, Viet D (18)
|
PVMSSC |
28.27 |
27.96 |
-0.31 |
| 23 |
Keeler, Nicholas W (16)
|
PVRMSC |
26.74 |
28.03 |
1.29 |
| 24 |
Oleson, Jack B (15)
|
PVTOLL |
27.87 |
28.11 |
0.24 |
| 25 |
Gabrielian, Leon N (18)
|
PVTOLL |
28.14 |
28.16 |
0.02 |
| 26 |
Tun, Ethan W (15)
|
PVNCAP |
26.62 |
28.18 |
1.56 |
| 26 |
Heath, Michael A (16)
|
PVUN |
|
28.18 |
- |
| 28 |
Braun, Zak H (16)
|
PVRMSC |
28.67 |
28.49 |
-0.18 |
| 29 |
Ament, Theodore T (15)
|
PVRMSC |
28.59 |
28.63 |
0.04 |
| 30 |
Greigg, Daniel (17)
|
PVRMSC |
28.84 |
28.65 |
-0.19 |
| 31 |
Venit, Michael (15)
|
PVPAC |
28.07 |
28.94 |
0.87 |
| 32 |
Vu, Christopher (16)
|
PVRMSC |
|
29.03 |
- |
| 33 |
Nagao, Yuki (18)
|
PVRMSC |
28.87 |
29.21 |
0.34 |
| 34 |
Lian, Daniel (15)
|
PVRMSC |
29.07 |
29.40 |
0.33 |
| 35 |
Chen, Alex M (16)
|
PVRMSC |
28.99 |
29.69 |
0.70 |
| 36 |
Wilbur, Eric J (16)
|
PVRMSC |
30.74 |
29.90 |
-0.84 |
| 37 |
Arcuri, Tobias F (15)
|
PVRMSC |
29.18 |
29.98 |
0.80 |
| 38 |
Neukam, Alexander L (16)
|
PVTIBU |
30.80 |
30.47 |
-0.33 |
| 39 |
Castaneda, Samuel (15)
|
PVRMSC |
29.84 |
31.08 |
1.24 |
| 40 |
Mueller, Matthew K (15)
|
PVRMSC |
31.24 |
31.21 |
-0.03 |
|
Iscoa, Nicolas A (17)
|
PVRMSC |
28.23 |
NS |
- |