| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rodeffer, Nyah A (8)
|
PVFAST |
26.16 |
22.45 |
-3.71 |
| 2 |
Lei, Angela (8)
|
PVMSSC |
23.19 |
22.49 |
-0.70 |
| 3 |
Mullen, Avery E (8)
|
PVLCL |
25.56 |
22.94 |
-2.62 |
| 4 |
Hammond, Sydney (8)
|
PVWEA |
24.93 |
23.51 |
-1.42 |
| 5 |
Chong, Sadie A (8)
|
PVTIBU |
25.22 |
23.65 |
-1.57 |
| 6 |
Sanogo, Amani C (8)
|
PVLCL |
|
23.77 |
- |
| 7 |
Johnson, Lauren G (8)
|
PVDCPR |
28.29 |
24.03 |
-4.26 |
| 8 |
Mott, Mathilda S (8)
|
PVDCPR |
25.03 |
24.07 |
-0.96 |
| 9 |
Brinkley-Tyson, Malaijah A (8)
|
PVDCPR |
23.80 |
24.19 |
0.39 |
| 10 |
Elliott, Rebecca C (8)
|
PVFAST |
24.69 |
24.22 |
-0.47 |
| 11 |
Burakiewicz, Imogen C (8)
|
PVLCL |
31.45 |
24.40 |
-7.05 |
| 12 |
Dickman, Emi K (8)
|
PVLCL |
|
24.84 |
- |
| 13 |
Batty, Kayla M (8)
|
PVPAC |
25.22 |
25.20 |
-0.02 |
| 14 |
Fouts, Emerson A (8)
|
PVDCPR |
23.75 |
25.26 |
1.51 |
| 15 |
Steele, Abigail M (8)
|
PVLCL |
|
25.53 |
- |
| 16 |
Korsten, Rosa (8)
|
PVLCL |
|
27.15 |
- |
| 17 |
Jones, Sofia K (8)
|
PVFAST |
27.32 |
27.63 |
0.31 |
| 18 |
Brittain, Nia K (8)
|
PVMSSC |
30.57 |
31.06 |
0.49 |
| 19 |
Zhang, Emily W (8)
|
PVMSSC |
|
38.32 |
- |
|
Fee, Sarah C (8)
|
PVDCPR |
28.94 |
NS |
- |