| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Basilio, Carter T (8)
|
PVNCAP |
|
43.80 |
- |
| 2 |
Velazquez, Oliver F (10)
|
PVPAC |
42.56 |
44.16 |
1.60 |
| 3 |
Hillman, Jared S (10)
|
PVPGPR |
45.01 |
45.59 |
0.58 |
| 4 |
Baptiste, Isaac C (9)
|
PVPGPR |
40.10 |
45.94 |
5.84 |
| 5 |
Williams, Ethyn P (10)
|
PVPAC |
58.29 |
50.54 |
-7.75 |
| 6 |
Qiao, Yirui (7)
|
PVRY |
48.36 |
51.27 |
2.91 |
| 7 |
Corbin Lewis, Jair N (10)
|
PVPGPR |
52.57 |
51.98 |
-0.59 |
| 8 |
Josephs, Alec M (9)
|
PVPGPR |
50.29 |
53.47 |
3.18 |
| 9 |
Lubis, Oliver A (9)
|
PVPAC |
|
55.35 |
- |
| 10 |
Avery, Harrison C (9)
|
PVHEAL |
57.24 |
56.66 |
-0.58 |
| 11 |
Koeser, Brayden (9)
|
PVWEA |
59.06 |
1:01.32 |
2.26 |
| 12 |
Hendershot, Thomas R (10)
|
PVPAC |
1:32.14 |
1:51.73 |
19.59 |
|
Sisul, Leo J (10)
|
PVHEAL |
48.70 |
NS |
- |
|
Andrade, Matthew A (9)
|
PVPAC |
49.85 |
NS |
- |
|
Lakanse, Fijinoluwa M (9)
|
PVPGPR |
51.85 |
NS |
- |