| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wexler, Hannah A (10)
|
PVNCAP |
34.46 |
34.03 |
-0.43 |
| 2 |
Kominski, Harper K (9)
|
PVNCAP |
37.81 |
38.54 |
0.73 |
| 3 |
Zhao, Alice R (10)
|
PVNCAP |
48.37 |
38.77 |
-9.60 |
| 4 |
Propper, Talia E (10)
|
PVNCAP |
43.62 |
38.83 |
-4.79 |
| 5 |
Zettler, Grace A (9)
|
PVNCAP |
48.07 |
42.01 |
-6.06 |
| 6 |
Hickey, Mia W (9)
|
PVNCAP |
42.87 |
44.33 |
1.46 |
| 7 |
Kim, Chaehyun (10)
|
PVTIBU |
49.26 |
45.01 |
-4.25 |
| 8 |
Bice, Tabitha Y (9)
|
PVPAC |
50.45 |
45.04 |
-5.41 |
| 9 |
Silbowitz, Dylan S (10)
|
PVJFD |
47.70 |
47.74 |
0.04 |
| 10 |
Luna, Olivia C (9)
|
PVJFD |
50.74 |
48.13 |
-2.61 |
| 11 |
Francis, Molly R (9)
|
PVPAC |
1:04.58 |
48.33 |
-16.25 |
| 12 |
Harrison, Evie S (9)
|
PVNCAP |
|
48.47 |
- |
| 13 |
Cooper, Ava (9)
|
PVPAC |
49.50 |
49.63 |
0.13 |
| 14 |
Britt, Christina N (10)
|
PVASTS |
1:03.58 |
50.76 |
-12.82 |
| 15 |
Breeden, Layla H (10)
|
PVNCAP |
|
52.30 |
- |
| 16 |
Kessler, Sarah L (9)
|
PVTOLL |
|
53.94 |
- |
| 17 |
Keneipp, Marissa D (10)
|
PVLIFE |
|
54.01 |
- |
| 18 |
Fong, Emma A (9)
|
PVJFD |
|
54.51 |
- |
|
Obeng, Sasha (9)
|
PVLIFE |
|
DQ |
- |
|
Kepler, Maya V (9)
|
PVJFD |
|
DQ |
- |
|
Ballard, Boe C (10)
|
PVTIBU |
|
DQ |
- |
|
Krieg, Rachel E (9)
|
PVJFD |
|
NS |
- |
|
Zossoungbo, Coralyn A (10)
|
PVTIBU |
|
NS |
- |