| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jungers, Megan K (14)
|
PVMACH |
4:58.38 |
5:00.31 |
1.93 |
| 2 |
Church, Abby L (14)
|
PVMACH |
5:06.81 |
5:02.64 |
-4.17 |
| 3 |
Schaefer, Amelia G (13)
|
PVMACH |
|
5:05.65 |
- |
| 4 |
Kleckner, Marissa J (14)
|
PVMACH |
|
5:08.83 |
- |
| 5 |
Walter, Catherine M (13)
|
PVMACH |
|
5:17.45 |
- |
| 6 |
Kasemsuwannakaeth, Paige (14)
|
PVMACH |
|
5:19.28 |
- |
| 7 |
McCullough, Ainsley E (13)
|
PVMACH |
|
5:34.14 |
- |
| 8 |
Ren, Emily Y (13)
|
PVMACH |
|
5:35.08 |
- |
| 9 |
Doody, Claire L (14)
|
PVMACH |
|
5:35.44 |
- |
| 10 |
McCullough, Teagan M (13)
|
PVMACH |
|
5:53.51 |
- |
| 11 |
Holmes, Lindsey G (13)
|
PVMACH |
|
5:56.12 |
- |
| 12 |
Raeder, Savannah J (13)
|
PVMACH |
6:29.11 |
6:23.87 |
-5.24 |
|
Aybar, Rachel M (13)
|
PVMACH |
|
DQ |
- |
|
Hall, Paige E (13)
|
PVMACH |
|
DQ |
- |