| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brown, Sophia V (12)
|
PVMACH |
59.28 |
59.27 |
-0.01 |
| 2 |
Chava, Megha N (12)
|
PVMACH |
1:01.57 |
1:00.98 |
-0.59 |
| 3 |
Stukus, Aleena J (11)
|
PVMACH |
1:01.00 |
1:02.11 |
1.11 |
| 4 |
Gray, Molly C (12)
|
PVMACH |
1:02.61 |
1:02.36 |
-0.25 |
| 5 |
Nguyen, Ava A (12)
|
PVMACH |
1:02.42 |
1:02.90 |
0.48 |
| 6 |
Connelly, Caitlin R (12)
|
PVMACH |
1:03.97 |
1:05.43 |
1.46 |
| 7 |
Krein, Olivia E (12)
|
PVMACH |
1:05.68 |
1:05.81 |
0.13 |
| 8 |
Schmidt, Kara L (11)
|
PVMACH |
1:04.66 |
1:06.31 |
1.65 |
| 9 |
Flickinger, Emma E (12)
|
PVMACH |
1:06.68 |
1:06.37 |
-0.31 |
| 10 |
Swanson, Aubrey A (11)
|
PVMACH |
1:07.82 |
1:07.02 |
-0.80 |
| 11 |
Yotmanee, Natasha S (12)
|
PVMACH |
1:06.27 |
1:07.53 |
1.26 |
| 12 |
Ross, Leah M (12)
|
PVMACH |
1:08.44 |
1:09.32 |
0.88 |
| 13 |
Norris, Riley E (12)
|
PVMACH |
1:10.37 |
1:09.87 |
-0.50 |
| 14 |
Jackson, Isabelle L (11)
|
PVMACH |
1:11.92 |
1:10.69 |
-1.23 |
| 15 |
Losinski, Kylie M (11)
|
PVMACH |
1:08.83 |
1:10.77 |
1.94 |
| 16 |
Johnson-Simmons, Brianna D (12)
|
PVMACH |
1:09.63 |
1:11.16 |
1.53 |
| 17 |
Haryanto, Karolina M (11)
|
PVMACH |
1:12.62 |
1:11.22 |
-1.40 |
| 18 |
McEnaney, Maddie V (11)
|
PVMACH |
1:11.14 |
1:11.41 |
0.27 |
| 19 |
Escamilla, Ana R (12)
|
PVMACH |
|
1:11.84 |
- |
| 20 |
Fredericks, Teah C (12)
|
PVMACH |
1:13.32 |
1:12.07 |
-1.25 |
| 21 |
Zuhoski, Olivia R (11)
|
PVMACH |
1:19.97 |
1:12.40 |
-7.57 |
| 22 |
Llames, Gemma L (12)
|
PVMACH |
1:14.05 |
1:13.88 |
-0.17 |
| 23 |
Valverde, Lily J (11)
|
PVMACH |
1:15.91 |
1:15.84 |
-0.07 |
| 24 |
Blachere, Josette T (11)
|
PVMACH |
1:18.64 |
1:16.16 |
-2.48 |
| 25 |
Buff, Reagan G (11)
|
PVMACH |
1:22.71 |
1:22.19 |
-0.52 |
| 26 |
Mathes, Maya N (11)
|
PVMACH |
|
1:27.08 |
- |
|
Ellis, Nisa A (12)
|
PVMACH |
57.58 |
NS |
- |