| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stapleton, Tallis M (8)
|
GUSHAC |
38.24 |
37.97 |
-0.27 |
| 2 |
Sosa, Lylah E (8)
|
GUSHAC |
38.46 |
39.00 |
0.54 |
| 3 |
Sosa, Lyza A (7)
|
GUSHAC |
40.90 |
40.21 |
-0.69 |
| 4 |
Valchar, Addley P (7)
|
GUSHAC |
43.12 |
40.83 |
-2.29 |
| 5 |
Fontenot, Kalyssa M (7)
|
GUSHAC |
|
46.93 |
- |
| 6 |
Schauss, Ingrid A (8)
|
GUAQUA |
52.82 |
47.73 |
-5.09 |
| 7 |
Haver, Leighton J (7)
|
GUSSS |
|
48.84 |
- |
| 8 |
Croniser, Abigail C (8)
|
GUSSS |
|
49.02 |
- |
| 9 |
Bonow, Blythe H (8)
|
GUAQUA |
|
51.06 |
- |
| 10 |
Matthews, Charlotte G (7)
|
GUSCAT |
|
55.35 |
- |
| 11 |
Pruneda, Alexandara E (5)
|
GUSSS |
|
58.86 |
- |
| 12 |
Xu, Suni (8)
|
GUSSS |
|
59.52 |
- |
| 13 |
Ellington, Olivia E (6)
|
GUSCAT |
|
1:00.49 |
- |
| 14 |
Cassady, Addison E (7)
|
GUAQUA |
1:03.66 |
1:02.05 |
-1.61 |
| 15 |
Carr, Quinlynne D (8)
|
GULAP |
|
1:04.58 |
- |
| 16 |
Koyfman, Emily S (7)
|
GUSSS |
|
1:08.37 |
- |
| 17 |
Johnson, Kaja L (7)
|
GUSSS |
|
1:17.67 |
- |
| 18 |
Patel, Sona R (7)
|
GUSSS |
|
1:22.15 |
- |