| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Maines, Keelyn J (8)
|
OHCY |
20.59 |
19.21 |
-1.38 |
| 2 |
Grant, Audriana R (8)
|
OHGMVY |
23.13 |
19.74 |
-3.39 |
| 3 |
Van Schoyck, Madison R (7)
|
OHCY |
22.79 |
19.76 |
-3.03 |
| 4 |
Crumback, Julia G (7)
|
OHCY |
22.40 |
21.14 |
-1.26 |
| 6 |
Stover, Teagan K (7)
|
OHCY |
22.51 |
21.84 |
-0.67 |
| 8 |
Cornele, Audrey (8)
|
OHGMVY |
22.09 |
22.24 |
0.15 |
| 9 |
Jansen, Isabella J (7)
|
OHCY |
23.21 |
22.36 |
-0.85 |
| 10 |
Appell, Katie N (8)
|
OHCY |
25.98 |
22.88 |
-3.10 |
| 11 |
Roberts, Karma M (8)
|
OHCFY |
23.91 |
23.08 |
-0.83 |
| 12 |
Boehm, Tabitha R (7)
|
OHCFY |
22.01 |
23.15 |
1.14 |
| 13 |
Guibord, Lucille C (8)
|
OHCY |
24.23 |
23.77 |
-0.46 |
| 14 |
Andress, Andie R (8)
|
OHCY |
22.78 |
23.98 |
1.20 |
| 15 |
Clapp, Riley A (7)
|
OHCY |
22.63 |
24.08 |
1.45 |
| 17 |
Brown, Reagan A (7)
|
OHCY |
25.23 |
24.28 |
-0.95 |
| 18 |
Knaley, Sophia A (8)
|
OHGMVY |
20.82 |
25.04 |
4.22 |
| 19 |
Guibord, Abigail D (8)
|
OHCY |
24.91 |
25.31 |
0.40 |
| 20 |
Siders, Kamery A (6)
|
OHCY |
28.73 |
25.49 |
-3.24 |
| 21 |
Beecroft, Tabitha J (8)
|
OHCFY |
29.70 |
25.70 |
-4.00 |
| 22 |
Farrell, Lanie G (7)
|
OHCY |
23.96 |
26.06 |
2.10 |
| 23 |
Slayton, Maya (7)
|
OHGMVY |
25.53 |
26.41 |
0.88 |
| 25 |
Davila Lopez, Allisson D (8)
|
OHCY |
30.46 |
28.88 |
-1.58 |
| 26 |
Newland, Welles Ayn S (8)
|
OHCY |
28.71 |
29.47 |
0.76 |
| 27 |
Triff, Bella V (7)
|
OHCY |
33.49 |
32.87 |
-0.62 |
| 28 |
Hensley, Olivia A (8)
|
OHCY |
32.19 |
33.32 |
1.13 |
| 29 |
Maines, Delaney L (6)
|
OHCY |
41.04 |
34.34 |
-6.70 |
| 30 |
Boyle, Dakota L (7)
|
OHCY |
34.08 |
35.83 |
1.75 |
| 31 |
Hastings, Penelope M (7)
|
OHCY |
41.62 |
45.40 |
3.78 |
|
Tenbusch, Kaitlyn A (7)
|
OHCY |
32.96 |
DQ |
- |
|
Hensley, Elianna J (8)
|
OHCY |
31.21 |
DQ |
- |
|
Beecroft, Gretchen E (5)
|
OHCFY |
31.76 |
DQ |
- |