| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cunningham, Jacqueline R (16)
|
OHCY |
2:25.69 |
2:21.77 |
-3.92 |
| 2 |
Meinking, Cecelia R (17)
|
OHCFY |
2:22.80 |
2:25.08 |
2.28 |
| 3 |
Tenbusch, Julia E (15)
|
OHCY |
2:26.41 |
2:25.61 |
-0.80 |
| 4 |
Kuertz, Maria E (15)
|
OHGMVY |
2:23.97 |
2:27.69 |
3.72 |
| 5 |
Kern, Abigail (16)
|
OHKEY |
2:36.03 |
2:29.22 |
-6.81 |
| 6 |
Gulczinski, Rachel A (15)
|
OHCFY |
2:31.27 |
2:31.22 |
-0.05 |
| 7 |
Lentine, Lillian C (17)
|
OHCY |
2:33.16 |
2:33.40 |
0.24 |
| 8 |
Wethington, Faith M (16)
|
OHCY |
2:49.95 |
2:34.33 |
-15.62 |
| 9 |
Cowell, Grace M (15)
|
OHKEY |
2:36.13 |
2:34.51 |
-1.62 |
| 10 |
Smith, Kaitlin (16)
|
OHKEY |
2:37.16 |
2:36.39 |
-0.77 |
| 11 |
Eckert, Cassandra M (16)
|
OHCFY |
2:32.71 |
2:39.92 |
7.21 |
| 12 |
Patterson, Jordan E (15)
|
OHCY |
2:40.75 |
2:41.02 |
0.27 |
| 13 |
Kuetemeyer, Gabriella R (15)
|
OHCY |
2:48.12 |
2:44.46 |
-3.66 |
| 14 |
Shaw, Stevie L (15)
|
OHCFY |
2:52.59 |
2:52.42 |
-0.17 |
| 15 |
Ballard, Hanna M (17)
|
OHCY |
3:05.62 |
3:00.42 |
-5.20 |
| 16 |
Townsend, Natalee E (16)
|
OHKEY |
3:20.55 |
3:03.13 |
-17.42 |
|
Deerhake, Jaden J (15)
|
OHCY |
2:46.40 |
DQ |
- |
|
Parry, Anna K (17)
|
OHCY |
2:39.10 |
DQ |
- |
|
Sundermann, Claire E (16)
|
OHCFY |
2:37.05 |
NS |
- |