| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Molina, Julia M (8)
|
OHLAKY |
56.94 |
47.56 |
-9.38 |
| 2 |
Spetz, Marlena A (7)
|
OHLAKY |
|
55.35 |
- |
| 3 |
Cukovecki, Siena R (8)
|
OHCY |
1:10.46 |
56.37 |
-14.09 |
| 4 |
Li, Xinran (8)
|
OHBASH |
56.52 |
57.36 |
0.84 |
| 5 |
Harnar, Sara K (8)
|
OHCY |
1:09.24 |
57.45 |
-11.79 |
| 6 |
Parks, Abigail M (8)
|
OHLAKY |
|
58.26 |
- |
| 7 |
Maines, Delaney L (8)
|
OHCY |
1:12.54 |
1:01.21 |
-11.33 |
| 8 |
Shoff, Maddison G (8)
|
OHBASH |
|
1:03.55 |
- |
| 9 |
McClung, Harper K (8)
|
OHCY |
|
1:10.89 |
- |
| 10 |
Schlessman, Ella A (8)
|
OHCY |
1:29.96 |
1:12.76 |
-17.20 |
| 11 |
Malavich, Greta J (7)
|
OHCY |
|
1:12.99 |
- |
| 12 |
Vaughn, Ellie G (7)
|
OHCY |
|
1:15.16 |
- |
| 13 |
Mullen, Emma G (8)
|
OHCY |
|
1:15.70 |
- |
| 14 |
Dyson, Evalyn F (8)
|
OHLAKY |
|
1:17.04 |
- |
| 15 |
Speed, Ruby J (6)
|
OHCY |
|
1:30.97 |
- |
|
Yao, Eileen S (8)
|
OHLAKY |
|
DQ |
- |
|
Nair, Anindita (7)
|
OHLAKY |
|
DQ |
- |
|
Robinson, Harper J (8)
|
OHCY |
|
DQ |
- |
|
Hogan, Jadyn E (7)
|
OHCY |
|
DQ |
- |
|
Azpiroz Galante, Nico (8)
|
OHBASH |
|
NS |
- |