| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shanks, Cory A (17)
|
GUSSS |
26.02 |
26.00 |
-0.02 |
| 2 |
Francis, Riley J (17)
|
NTLAC |
26.16 |
26.44 |
0.28 |
| 3 |
Truong, Kyanh (17)
|
STWHAC |
26.16 |
26.54 |
0.38 |
| 4 |
Bodenstab, Amelia N (14)
|
STASC |
28.55 |
27.30 |
-1.25 |
| 5 |
Fleming, Anastasia E (17)
|
STWHAC |
26.42 |
27.40 |
0.98 |
| 6 |
Bachl, Lily A (16)
|
STWHAC |
27.16 |
27.42 |
0.26 |
| 7 |
Schellenger, Sienna E (16)
|
NTLAC |
26.88 |
27.68 |
0.80 |
| 8 |
Basler, Alia R (16)
|
STUN-0 |
26.96 |
27.80 |
0.84 |
| 9 |
Bulseco, Kayla L (15)
|
GUSSS |
28.29 |
27.99 |
-0.30 |
| 10 |
Wu, Kaylee M (15)
|
STWHAC |
27.86 |
28.04 |
0.18 |
| 11 |
Lanigan, Caitlin E (16)
|
GUTWST |
27.67 |
28.09 |
0.42 |
| 12 |
Phillips, Charlize L (16)
|
CCCLOV |
29.11 |
28.35 |
-0.76 |
| 13 |
Glasgow, Isabella M (18)
|
GUSSS |
28.35 |
28.59 |
0.24 |
| 14 |
Garriss, Olivia M (16)
|
GUTWST |
28.62 |
28.89 |
0.27 |
| 15 |
Schwendinger, Reagan J (15)
|
NTLAC |
31.47 |
29.33 |
-2.14 |
| 16 |
Kruzel, Peighton J (14)
|
NTLAC |
30.62 |
30.01 |
-0.61 |
| 17 |
True, Lauren E (15)
|
NTLAC |
30.51 |
30.23 |
-0.28 |
| 18 |
Smith, Elizabeth A (24)
|
STWHAC |
29.57 |
30.26 |
0.69 |
| 19 |
Kobukai, Koto (10)
|
NTLAC |
34.86 |
31.23 |
-3.63 |
| 20 |
Kruzel, Parker R (11)
|
NTLAC |
36.84 |
31.73 |
-5.11 |
| 21 |
Zlinsky, Zorie A (12)
|
NTLAC |
35.24 |
32.00 |
-3.24 |
| 22 |
Kobukai, Sato (13)
|
NTLAC |
31.63 |
32.11 |
0.48 |
| 23 |
Javier, Madison M (11)
|
NTLAC |
32.52 |
32.83 |
0.31 |
| 24 |
Jimenez, Sophia (11)
|
NTLAC |
35.63 |
33.96 |
-1.67 |
| 25 |
Masters, Cassidy K (11)
|
NTLAC |
39.02 |
33.97 |
-5.05 |
| 26 |
Ross, Callista A (12)
|
NTLAC |
34.97 |
34.03 |
-0.94 |
|
Spencer, Macy L (12)
|
NTLAC |
37.35 |
DQ |
- |
|
Mathews, Georgianna E (18)
|
GURICE |
27.96 |
NS |
- |