| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
D'Spain, Brady M (12)
|
NTUN |
40.83 |
36.38 |
-4.45 |
| 2 |
Pham, Ryan D (12)
|
NTESP |
|
40.81 |
- |
| 3 |
Wing, Dylan C (12)
|
NTESP |
45.70 |
44.65 |
-1.05 |
| 4 |
Bartell, Brendan A (12)
|
NTESP |
|
45.53 |
- |
| 5 |
Putty, Jacob C (12)
|
NTMARS |
|
46.67 |
- |
| 6 |
Caruso, Kamren J (10)
|
NTATAC |
47.77 |
47.92 |
0.15 |
| 7 |
Tjio, Samuel (12)
|
NTMARS |
50.88 |
49.09 |
-1.79 |
| 8 |
Parker, Jayden V (8)
|
NTMTRO |
52.49 |
50.87 |
-1.62 |
| 9 |
Reichert, Ian N (9)
|
NTATAC |
55.40 |
57.16 |
1.76 |
| 10 |
Sayyid, Kian F (10)
|
NTATAC |
|
58.39 |
- |
| 11 |
Sureka, Sheel (8)
|
NTATAC |
1:00.89 |
1:00.22 |
-0.67 |
| 12 |
Fox, Dylan A (9)
|
NTESP |
57.78 |
1:01.59 |
3.81 |
|
Case, Scott W (6)
|
NTESP |
|
DQ |
- |
|
Nichols, William C (6)
|
NTESP |
|
DQ |
- |
|
Lugo, Sergio R (9)
|
NTMARS |
57.55 |
DQ |
- |
|
Wang, Jason (9)
|
NTMARS |
|
DQ |
- |
|
Womeldorph, Christopher M (8)
|
NTMARS |
|
DQ |
- |
|
Abshire, Andrew (11)
|
NTATAC |
56.02 |
NS |
- |