| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Francis, Riley J (17)
|
NTLAC |
56.09 |
56.84 |
0.75 |
| 2 |
Alas, Isabella M (16)
|
GUDADS |
57.66 |
58.22 |
0.56 |
| 3 |
Bodenstab, Amelia N (14)
|
STASC |
1:04.14 |
58.92 |
-5.22 |
| 4 |
Bachl, Lily A (16)
|
STWHAC |
58.98 |
59.48 |
0.50 |
| 5 |
Phillips, Charlize L (16)
|
CCCLOV |
1:00.39 |
1:00.19 |
-0.20 |
| 6 |
Basler, Alia R (16)
|
STUN-0 |
59.78 |
1:00.91 |
1.13 |
| 7 |
Guffey, Ella M (16)
|
CCCLOV |
1:01.34 |
1:02.80 |
1.46 |
| 8 |
Carey, Lexi J (15)
|
MTBAC |
1:07.51 |
1:03.75 |
-3.76 |
| 9 |
Schwendinger, Reagan J (15)
|
NTLAC |
1:10.34 |
1:04.45 |
-5.89 |
| 10 |
Kobukai, Koto (10)
|
NTLAC |
1:13.15 |
1:06.41 |
-6.74 |
| 11 |
Matkovich, Bryanna M (18)
|
NTUN |
1:03.54 |
1:08.54 |
5.00 |
| 12 |
Kruzel, Parker R (11)
|
NTLAC |
1:20.87 |
1:09.55 |
-11.32 |
| 13 |
Ross, Callista A (12)
|
NTLAC |
1:16.11 |
1:13.41 |
-2.70 |
| 14 |
Masters, Cassidy K (11)
|
NTLAC |
1:26.98 |
1:14.91 |
-12.07 |
|
Mathews, Georgianna E (18)
|
GURICE |
59.87 |
NS |
- |