| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carlson, Jaelle S (18)
|
NTMTRO |
5:38.63 |
5:26.89 |
-11.74 |
| 2 |
Walker, Lorelai G (15)
|
NTTRAC |
5:39.09 |
5:34.11 |
-4.98 |
| 3 |
Dangelmaier, Gianna M (14)
|
NTMARS |
5:35.73 |
5:43.59 |
7.86 |
| 4 |
Alberts, Payton F (17)
|
NTMTRO |
5:46.16 |
5:50.47 |
4.31 |
| 5 |
Louden, Ryan M (14)
|
NTMTRO |
5:39.50 |
5:50.85 |
11.35 |
| 6 |
Henson, Molly J (17)
|
NTMARS |
5:57.75 |
6:01.58 |
3.83 |
| 7 |
Griffith, Rachel R (14)
|
NTPAC |
6:37.97 |
6:08.33 |
-29.64 |
| 8 |
Gadsby, Audrey C (13)
|
STUN |
6:09.38 |
6:09.85 |
0.47 |
| 9 |
Nichols, Ella K (13)
|
NTESP |
6:27.73 |
6:17.16 |
-10.57 |
| 10 |
Wright, Jessie C (16)
|
NTMTRO |
6:24.00 |
6:23.22 |
-0.78 |
| 12 |
Olivieri, Breanna Q (14)
|
NTMTRO |
6:12.98 |
6:30.97 |
17.99 |
| 13 |
Giesinger, Georgia G (12)
|
NTLTSD |
6:40.11 |
6:33.36 |
-6.75 |
| 14 |
McFadden, Madalynn G (12)
|
NTPAC |
|
7:09.38 |
- |
| 15 |
Estes, Alexandra G (14)
|
NTMTRO |
|
7:25.16 |
- |
|
Barrington, Sofia I (13)
|
NTMARS |
5:44.23 |
NS |
- |
|
Reynolds, Lena I (13)
|
NTLTSD |
5:35.57 |
NS |
- |
|
Reynolds, Brenna L (11)
|
NTLTSD |
6:53.62 |
NS |
- |
|
Tufail, Shanzay F (11)
|
NTUN |
6:43.09 |
NS |
- |