| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blanford, Joey (8)
|
KYNEY |
20.72 |
20.99 |
0.27 |
| 2 |
Brown, Isabella C (8)
|
KYBYB |
23.40 |
22.51 |
-0.89 |
| 3 |
Mayer, Josie (8)
|
KYNLY |
21.26 |
22.57 |
1.31 |
| 4 |
Seastrand-Stone, Maci E (7)
|
KYBYB |
23.88 |
22.67 |
-1.21 |
| 5 |
Bohannon, Natalie J (8)
|
KYBYB |
26.47 |
23.30 |
-3.17 |
| 6 |
Godthaab, Maggie (8)
|
KYNEY |
21.09 |
23.70 |
2.61 |
| 7 |
Sanchez, Nicole A (8)
|
KYBYB |
23.44 |
24.19 |
0.75 |
| 8 |
Humphrey, Adelyn (7)
|
KYNEY |
23.50 |
24.20 |
0.70 |
| 9 |
Jenkins, Fenley (8)
|
KYNEY |
24.40 |
24.66 |
0.26 |
| 10 |
Wong, Jolene Y (8)
|
KYNLY |
22.55 |
24.72 |
2.17 |
| 11 |
Corder, Elizabeth (8)
|
KYTYP |
21.66 |
24.76 |
3.10 |
| 12 |
Giles, Ellie (8)
|
KYNEY |
22.73 |
25.08 |
2.35 |
| 13 |
Shone, Livia (8)
|
KYPBCY |
24.74 |
25.23 |
0.49 |
| 14 |
Pryor, Camden B (8)
|
KYBYB |
24.51 |
25.38 |
0.87 |
| 15 |
Maynard, Kenley P (7)
|
KYAAY |
23.77 |
25.76 |
1.99 |
| 16 |
Tovar, Annie (8)
|
KYNLY |
24.28 |
25.83 |
1.55 |
| 17 |
Miller, Lexie J (7)
|
KYAAY |
25.32 |
26.48 |
1.16 |
| 18 |
Cornely, Olivia (8)
|
KYNEY |
26.21 |
26.51 |
0.30 |
| 19 |
Cotter, Eabha (7)
|
KYPBCY |
27.91 |
26.66 |
-1.25 |
| 20 |
Smotherman, Elizabeth A (8)
|
KYBYB |
31.92 |
26.95 |
-4.97 |
| 21 |
Agrinsoni, Asia (8)
|
KYNEY |
27.08 |
27.14 |
0.06 |
| 22 |
MacCarthy, Madison L (8)
|
KYBYB |
41.78 |
27.89 |
-13.89 |
| 23 |
Duncan, Katherine H (7)
|
KYBYB |
27.76 |
28.03 |
0.27 |
| 24 |
Koch, Lucykate (8)
|
KYNEY |
26.23 |
29.40 |
3.17 |
| 25 |
Florence, Caitlyn (8)
|
KYLYSC |
35.20 |
29.95 |
-5.25 |
| 26 |
Gooch, Delilah L (7)
|
KYBYB |
34.41 |
31.83 |
-2.58 |
| 27 |
Atwell, Jacqueline (7)
|
KYTYP |
33.16 |
35.14 |
1.98 |
| 28 |
Spires, Mia (7)
|
KYNLY |
|
37.38 |
- |
| 29 |
Mauck, Adeline (7)
|
KYTYP |
35.49 |
38.26 |
2.77 |
|
Tejeda-Flores, Lia (7)
|
KYTYP |
|
DQ |
- |