| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Osada, Elizabeth R (9)
|
VAACAC |
44.53 |
36.38 |
-8.15 |
| 2 |
Dunham, Julia E (10)
|
VAGATR |
49.88 |
37.71 |
-12.17 |
| 3 |
Scherer, Hunter L (10)
|
VAACAC |
43.98 |
37.97 |
-6.01 |
| 4 |
Thompson, Isabelle R (10)
|
VAGATR |
47.08 |
39.15 |
-7.93 |
| 5 |
Scherer, Anne T (10)
|
VAACAC |
38.06 |
39.42 |
1.36 |
| 6 |
Reedy, Carly S (10)
|
VAGATR |
47.05 |
40.16 |
-6.89 |
| 7 |
Hooke, Alison P (9)
|
VAGATR |
1:00.99 |
40.44 |
-20.55 |
| 8 |
Keller, Madison G (10)
|
VAGATR |
52.05 |
41.09 |
-10.96 |
| 9 |
Bing, Rachael C (10)
|
VAGATR |
56.98 |
43.36 |
-13.62 |
| 10 |
Abdulaeva, Nicole (10)
|
VAACST |
|
51.21 |
- |
| 11 |
McCardle, Reese (9)
|
VAACAC |
45.59 |
51.83 |
6.24 |
| 12 |
Moseley, Kaitlyn S (9)
|
VAGATR |
1:07.80 |
57.66 |
-10.14 |
|
Douglas, Adeline M (10)
|
VAGATR |
55.66 |
DQ |
- |
|
Barclay, Sydney M (10)
|
VAACAC |
|
NS |
- |
|
Trella-Luedtke, Isabel G (10)
|
VAACST |
|
NS |
- |