| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reyes, Sara M (10)
|
NJRSA |
40.98 |
39.19 |
-1.79 |
| 2 |
Yao, Mulan (9)
|
NJSCAR |
48.10 |
41.65 |
-6.45 |
| 3 |
Vasavada, Reyna (9)
|
NJSCAR |
47.08 |
43.53 |
-3.55 |
| 4 |
Bhagaloo, Nataasha D (10)
|
NJNJB |
49.89 |
44.45 |
-5.44 |
| 5 |
Zheng, Kexin (8)
|
NJSCAR |
45.87 |
44.58 |
-1.29 |
| 6 |
Sawyer, Alison R (9)
|
NJSCAR |
43.38 |
46.21 |
2.83 |
| 7 |
Nguyen-Satchkov, Natalie (8)
|
NJSCAR |
44.20 |
46.42 |
2.22 |
| 8 |
Dutzi, Julia M (9)
|
NJNJB |
45.60 |
46.60 |
1.00 |
| 9 |
Konuganti, Anvitha (10)
|
NJSCAR |
46.97 |
48.87 |
1.90 |
| 10 |
Yeh, Allison (9)
|
NJSCAR |
47.97 |
49.49 |
1.52 |
| 11 |
Kim, Caylee J (9)
|
NJSCAR |
50.22 |
51.04 |
0.82 |
| 12 |
Turner, Caroline R (9)
|
NJNJB |
47.60 |
51.62 |
4.02 |
| 13 |
Lauber, Fiona J (9)
|
NJSCAR |
47.24 |
53.50 |
6.26 |
|
Adams, Kyleigh A (10)
|
NJDESC |
48.30 |
DQ |
- |
|
Wos, Giovanna P (10)
|
NJRSA |
45.22 |
DQ |
- |