| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Riddle, Stephen (9)
|
GUAGS |
48.52 |
43.03 |
-5.49 |
| 2 |
Shrihari, Virat (7)
|
GUAGS |
48.06 |
44.54 |
-3.52 |
| 3 |
Kimmel, August J (10)
|
GURICE |
46.85 |
45.44 |
-1.41 |
| 4 |
Miksenas, Jonas P (10)
|
GUKATY |
49.22 |
47.24 |
-1.98 |
| 5 |
Jones, Ayden M (8)
|
GUAMBU |
1:00.46 |
48.72 |
-11.74 |
| 6 |
Sprinkles, Jaxson J (8)
|
GURICE |
1:00.05 |
49.41 |
-10.64 |
| 7 |
Denton, Oliver P (8)
|
GUPACK |
51.73 |
49.67 |
-2.06 |
| 8 |
Nielander, Christopher T (9)
|
GUKATY |
51.40 |
50.86 |
-0.54 |
| 9 |
Chouliaris, Michael P (8)
|
GUKATY |
|
51.91 |
- |
| 10 |
Burnette, Stellan (10)
|
GUAMBU |
49.53 |
53.11 |
3.58 |
| 11 |
Liu, David J (8)
|
GUKATY |
1:00.97 |
53.74 |
-7.23 |
| 12 |
Kiyashchenko, Efim (7)
|
GULIFE |
1:06.20 |
59.54 |
-6.66 |
| 13 |
Wildermuth, Jack R (8)
|
GUPACK |
1:09.00 |
1:00.29 |
-8.71 |
| 14 |
Pardo, Isaac (8)
|
GUKATY |
1:02.67 |
1:00.74 |
-1.93 |
| 15 |
Zhang, Ryan (8)
|
GURICE |
|
1:10.02 |
- |
| 16 |
Wang, Hongyi (8)
|
GUKATY |
|
1:10.92 |
- |
|
Liu, Xinrui (9)
|
GUAGS |
1:06.81 |
DQ |
- |