| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aragon Calzada, Luciana (8)
|
GUMAC |
39.63 |
39.41 |
-0.22 |
| 2 |
Yang, Chloe Y (7)
|
GUSSAN |
43.23 |
39.97 |
-3.26 |
| 3 |
Bhakta, Aniya K (8)
|
GUSHRK |
49.40 |
42.47 |
-6.93 |
| 4 |
Oquendo, Jorlaney M (8)
|
GUPACK |
42.71 |
42.77 |
0.06 |
| 5 |
McDaniel, Kenley E (8)
|
GUMAC |
49.11 |
43.19 |
-5.92 |
| 6 |
Masch, Bianca D (8)
|
GUKATY |
44.86 |
44.51 |
-0.35 |
| 7 |
Griffin-Coyle, Addyson G (7)
|
GUMAC |
47.88 |
45.76 |
-2.12 |
| 8 |
Treadaway, Caeleigh R (8)
|
GUMAC |
50.61 |
47.94 |
-2.67 |
| 9 |
Reams, Piper J (8)
|
GUPACE |
54.29 |
48.83 |
-5.46 |
| 10 |
Smyers, Lillie R (7)
|
GUKATY |
53.22 |
48.90 |
-4.32 |
| 11 |
Knapp, Sofi (7)
|
GUMAC |
|
48.95 |
- |
| 12 |
Skinner, Sarah E (8)
|
GUMAC |
52.24 |
52.03 |
-0.21 |
| 13 |
Kumar, Saakshi (8)
|
GUKATY |
48.36 |
52.21 |
3.85 |
| 14 |
Botens, Phoebe H (8)
|
GUKATY |
56.87 |
52.27 |
-4.60 |
| 15 |
Zhang, Elizabeth C (6)
|
GUKATY |
|
58.40 |
- |
| 16 |
Rosebush, Emilee A (5)
|
GUMAC |
1:01.40 |
1:13.62 |
12.22 |