| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moss, Aidan T (12)
|
VALY |
32.22 |
32.97 |
0.75 |
| 2 |
Cunningham, Colton R (11)
|
VAVABR |
35.42 |
33.57 |
-1.85 |
| 3 |
Jaskot, Sutton C (11)
|
VACA-Y |
32.96 |
33.75 |
0.79 |
| 4 |
Litton, William (11)
|
VACA-Y |
37.36 |
34.13 |
-3.23 |
| 6 |
Kirby, Colt A (12)
|
VALY |
34.70 |
34.75 |
0.05 |
| 7 |
Manning, Grant W (12)
|
VALY |
35.39 |
35.53 |
0.14 |
| 8 |
Gustafson, Daniel L (11)
|
VALY |
35.03 |
36.18 |
1.15 |
| 9 |
Leger, Noah J (11)
|
VALY |
36.66 |
36.67 |
0.01 |
| 11 |
Barksdale, Jackson P (12)
|
VALY |
42.16 |
38.31 |
-3.85 |
| 12 |
Fisher, Garrick G (12)
|
VACA-Y |
40.64 |
38.55 |
-2.09 |
| 13 |
Beauchamp, William H (12)
|
VAFCFY |
41.57 |
38.60 |
-2.97 |
| 14 |
Eakkhattiyakorn, Tyler (12)
|
VALY |
42.09 |
40.40 |
-1.69 |
| 15 |
Cox, Clayton H (11)
|
VALY |
39.94 |
40.87 |
0.93 |
| 16 |
Thomas, Brandon C (12)
|
VALY |
45.05 |
41.24 |
-3.81 |
| 19 |
Koscielny, Jefferson P (12)
|
VALY |
47.46 |
45.48 |
-1.98 |
| 20 |
Berkley, William J (12)
|
VALY |
49.78 |
45.53 |
-4.25 |
|
Trosper, Eli T (12)
|
VALY |
|
DQ |
- |
|
Moore, Julius O (11)
|
VALY |
42.85 |
NS |
- |
|
Thomas, Brodie M (12)
|
VALY |
50.03 |
NS |
- |
|
Loura, Paul D (11)
|
VACA-Y |
35.75 |
NS |
- |