| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fleenor, Anna Clare C (14)
|
VASFY |
54.04 |
56.00 |
1.96 |
| 2 |
Eckert, Ellisa M (14)
|
VALY |
58.16 |
59.37 |
1.21 |
| 3 |
Murie, Rachel H (14)
|
VASMAC |
58.21 |
59.42 |
1.21 |
| 4 |
Wagner, Kristen L (14)
|
VASMAC |
|
1:00.94 |
- |
| 5 |
Johnson, Paris A (14)
|
VACYAC |
59.47 |
1:01.03 |
1.56 |
| 6 |
Stebbins, Kayleigh G (14)
|
VACYAC |
1:02.04 |
1:02.58 |
0.54 |
| 7 |
Burnsed, Adaire M (13)
|
VACYAC |
1:01.37 |
1:03.97 |
2.60 |
| 9 |
Nguyen, Caitlin J (14)
|
VACYAC |
1:04.54 |
1:06.02 |
1.48 |
| 10 |
Hatfield, Lindsay L (13)
|
VALY |
1:10.47 |
1:11.78 |
1.31 |
| 11 |
Ford, Grace c (14)
|
VACYAC |
1:09.31 |
1:11.98 |
2.67 |
| 12 |
Hughes, Savannah G (13)
|
WVYSWV |
1:09.08 |
1:12.62 |
3.54 |
| 13 |
Huffmyer, Jenna E (13)
|
VACYAC |
1:13.11 |
1:13.34 |
0.23 |
| 14 |
Farabaugh, Kristen N (13)
|
VACYAC |
1:13.28 |
1:14.34 |
1.06 |
| 18 |
Baker, Sidney M (14)
|
VALY |
1:16.14 |
1:16.10 |
-0.04 |
| 19 |
Maddox, Karsen A (13)
|
VALY |
1:23.73 |
1:17.87 |
-5.86 |