| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ryan, Margarita M (21)
|
VAUN |
31.35 |
29.61 |
-1.74 |
| 2 |
Keiner, Payton R (20)
|
VALIB |
29.73 |
30.20 |
0.47 |
| 3 |
Haines, Shelby E (19)
|
VALIB |
30.66 |
32.82 |
2.16 |
| 4 |
Sheng, Anna (17)
|
VAHOKI |
33.80 |
34.21 |
0.41 |
| 5 |
Tiller, Anne-Elaine (18)
|
VALY |
34.82 |
36.44 |
1.62 |
| 6 |
Warren, Grace K (15)
|
VALY |
37.13 |
36.86 |
-0.27 |
| 7 |
Russell, Caroline H (15)
|
VALY |
39.10 |
37.19 |
-1.91 |
| 8 |
Plogger, Alexis N (18)
|
VALY |
36.48 |
38.13 |
1.65 |
| 9 |
Thompson, Caraline E (15)
|
VAHOKI |
42.58 |
38.77 |
-3.81 |
| 10 |
Walker, Abigail E (15)
|
VALY |
|
38.92 |
- |
| 11 |
Dodge, Sophie G (15)
|
VALY |
40.30 |
39.11 |
-1.19 |
| 12 |
Hansen, Emily C (15)
|
VALY |
|
39.25 |
- |
| 13 |
Russell, Emma K (19)
|
VALY |
38.34 |
39.74 |
1.40 |
| 14 |
Farabaugh, Kristen N (15)
|
VACYAC |
43.33 |
43.83 |
0.50 |
| 15 |
Skinner, Ava M (17)
|
VALY |
|
50.79 |
- |
|
Haise, Sophie A (16)
|
VAACAC |
34.06 |
NS |
- |