| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eakkhattiyakorn, Tyler (12)
|
VALY |
37.58 |
36.66 |
-0.92 |
| 2 |
Gustafson, Daniel L (11)
|
VALY |
37.61 |
36.79 |
-0.82 |
| 3 |
Fisher, Garrick G (12)
|
VACA-Y |
38.28 |
38.88 |
0.60 |
| 4 |
Moss, Aidan T (12)
|
VALY |
42.07 |
42.05 |
-0.02 |
| 5 |
Barksdale, Jackson P (12)
|
VALY |
45.31 |
43.57 |
-1.74 |
| 6 |
Kirby, Colt A (12)
|
VALY |
43.15 |
43.93 |
0.78 |
| 7 |
Woodruff, Austin C (11)
|
VALY |
41.27 |
44.00 |
2.73 |
| 8 |
Beauchamp, William H (12)
|
VAFCFY |
41.84 |
44.31 |
2.47 |
| 9 |
Jaskot, Sutton C (11)
|
VACA-Y |
46.11 |
44.39 |
-1.72 |
| 11 |
McKenney, Indiana A (11)
|
VACA-Y |
46.33 |
46.14 |
-0.19 |
| 12 |
Thomas, Brandon C (12)
|
VALY |
47.69 |
46.25 |
-1.44 |
| 15 |
Berkley, William J (12)
|
VALY |
52.64 |
52.42 |
-0.22 |
|
Heil, Max V (11)
|
VACA-Y |
47.71 |
DQ |
- |
|
Loura, Paul D (11)
|
VACA-Y |
45.96 |
NS |
- |
|
Thomas, Brodie M (12)
|
VALY |
58.83 |
NS |
- |
|
Cunningham, Colton R (11)
|
VAVABR |
44.99 |
NS |
- |