| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Joslin, Shelby W (11)
|
GUBTA |
3:17.15 |
3:02.86 |
-14.29 |
| 2 |
Strickland, Emma K (11)
|
GUKATY |
3:03.59 |
3:03.48 |
-0.11 |
| 3 |
Jones, Lynsie A (11)
|
GUMAC |
3:22.57 |
3:19.24 |
-3.33 |
| 4 |
Shelton, Gracie L (11)
|
GUSSAN |
3:44.82 |
3:19.93 |
-24.89 |
| 5 |
Nitin, Kashvi (11)
|
GUFCST |
3:23.75 |
3:20.47 |
-3.28 |
| 6 |
Gumban, Lyra A (11)
|
GUFCST |
|
3:23.08 |
- |
| 7 |
Badeaux, Hailey (12)
|
GUESA |
|
3:26.21 |
- |
| 8 |
Loyola, Azel Rianna M (12)
|
GUFCST |
|
3:26.59 |
- |
| 9 |
Mathis, Mollie E (11)
|
GUFCST |
3:52.79 |
3:29.47 |
-23.32 |
| 10 |
Chen, Carrie (11)
|
GUFCST |
|
3:30.65 |
- |
| 11 |
Ramos, Alaina B (11)
|
GUFCST |
3:57.70 |
3:30.90 |
-26.80 |
| 12 |
Han, Elizabeth (11)
|
GUFCST |
4:14.00 |
3:44.96 |
-29.04 |
| 13 |
Morter, Jada K (11)
|
GUSSAN |
4:53.46 |
3:57.30 |
-56.16 |
| 14 |
Lopez DE LA Rosa, Victoria (11)
|
GUKATY |
|
4:10.51 |
- |