| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deffet, Cathryn E (10)
|
GUKATY |
2:55.23 |
2:53.00 |
-2.23 |
| 2 |
Luo, Michelle J (9)
|
GUAGS |
2:57.58 |
2:56.20 |
-1.38 |
| 3 |
White, Carlie R (10)
|
GUCATS |
3:37.75 |
2:59.32 |
-38.43 |
| 4 |
Laubsch, Giada G (9)
|
GUMAC |
3:03.52 |
3:07.88 |
4.36 |
| 5 |
Rick, Kyndal M (10)
|
GUCATS |
3:36.69 |
3:09.52 |
-27.17 |
| 6 |
Leonard, Kinley E (9)
|
GUMAC |
3:27.55 |
3:10.33 |
-17.22 |
| 7 |
Fan, Jessica (9)
|
GUKATY |
|
3:14.62 |
- |
| 8 |
Kleiman, Grace D (10)
|
GUKATY |
3:21.44 |
3:20.09 |
-1.35 |
| 9 |
Brown, Emily E (9)
|
GUMARC |
|
3:21.01 |
- |
| 10 |
Leonard, Kilynn N (10)
|
GUMAC |
3:32.51 |
3:22.84 |
-9.67 |
| 11 |
Trinh, Emily M (10)
|
GUSSAN |
3:36.74 |
3:25.54 |
-11.20 |
| 12 |
Kim, Kelly (9)
|
GUKATY |
|
3:28.54 |
- |
| 13 |
Menasria, Narjis (9)
|
GUFCST |
4:16.98 |
3:58.32 |
-18.66 |
| 14 |
Lopez DE LA Rosa, Luna N (9)
|
GUKATY |
4:13.24 |
4:01.30 |
-11.94 |
| 15 |
Zepeda-Otalora, Camila J (9)
|
GUKATY |
|
4:06.18 |
- |
| 16 |
Elizarraras, Emma I (10)
|
GUCATS |
|
4:11.60 |
- |
| 17 |
Skinner, Sarah E (9)
|
GUMAC |
4:58.87 |
4:19.97 |
-38.90 |
|
Schroeder, Madison A (9)
|
GUKATY |
2:59.81 |
NS |
- |
|
Brown, Laura E (10)
|
GUBTA |
|
NS |
- |