| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Satterfield, Nyah (8)
|
GUMAC |
43.61 |
42.37 |
-1.24 |
| 2 |
Rosebush, Allie L (8)
|
GUMAC |
43.88 |
44.78 |
0.90 |
| 3 |
Laubsch, Giada G (8)
|
GUMAC |
44.81 |
45.52 |
0.71 |
| 4 |
Aragon Ocampo, Luciana (8)
|
GUMAC |
51.03 |
48.74 |
-2.29 |
| 5 |
Shelton, Allison M (8)
|
GUSSAN |
|
49.05 |
- |
| 6 |
Leonard, Kinley E (8)
|
GUMAC |
52.33 |
50.52 |
-1.81 |
| 7 |
Dristas, Emma A (8)
|
GUMAC |
55.09 |
55.13 |
0.04 |
| 8 |
Skinner, Sarah E (8)
|
GUMAC |
59.11 |
58.26 |
-0.85 |
| 9 |
Scribner, Gabriella K (8)
|
GUSSAN |
1:07.41 |
59.93 |
-7.48 |
| 10 |
Metzger, Everly G (8)
|
GUSSAN |
|
1:06.82 |
- |
|
Brown, Emily E (8)
|
GUMARC |
54.63 |
NS |
- |
|
LeBlanc, Sophia A (8)
|
GUSSAN |
45.68 |
NS |
- |