| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rayson, Liam C (15)
|
PVMACH |
36.27 |
27.98 |
-8.29 |
| 2 |
Cantrell, Nicolas B (18)
|
PVMACH |
|
28.68 |
- |
| 3 |
Goriachev, Aleksandr Y (15)
|
PVMACH |
|
28.72 |
- |
| 4 |
Cobb, Benham Z (17)
|
PVMACH |
31.40 |
28.88 |
-2.52 |
| 5 |
Rushforth, James T (16)
|
PVMACH |
|
29.08 |
- |
| 6 |
Christofferson, Andrew G (17)
|
PVMACH |
37.16 |
29.38 |
-7.78 |
| 7 |
Rieger, Annika L (14)
|
PVMACH |
31.63 |
30.09 |
-1.54 |
| 8 |
Christofferson, Nicolas S (15)
|
PVMACH |
35.52 |
31.00 |
-4.52 |
| 9 |
Spurrier, Nathan J (15)
|
PVMACH |
40.72 |
32.53 |
-8.19 |
| 10 |
Chua, Daniel S (15)
|
PVMACH |
39.34 |
32.79 |
-6.55 |
| 11 |
Walker, Shay E (18)
|
PVNCAP |
33.93 |
32.83 |
-1.10 |
| 12 |
Kauffman, Matthew G (13)
|
PVMACH |
34.47 |
32.88 |
-1.59 |
| 13 |
Kass, Morgan L (13)
|
PVMACH |
33.65 |
33.39 |
-0.26 |
| 14 |
Taylormoore, Samantha L (15)
|
PVMACH |
|
33.44 |
- |
| 15 |
Schone, Liliana C (17)
|
PVMACH |
35.75 |
33.57 |
-2.18 |
| 16 |
Shifflett, Brooke S (13)
|
PVNCAP |
47.32 |
35.15 |
-12.17 |
| 17 |
McDonald, Peyton E (13)
|
PVNCAP |
38.00 |
35.80 |
-2.20 |
| 17 |
Woodside, Nina K (13)
|
PVNCAP |
42.02 |
35.80 |
-6.22 |
| 19 |
Buehler, Taylor G (13)
|
PVNCAP |
38.85 |
36.22 |
-2.63 |
| 20 |
Bonnaire, Julia R (13)
|
PVNCAP |
43.93 |
39.63 |
-4.30 |
| 21 |
Howard, Marshall T (13)
|
PVNCAP |
|
43.76 |
- |