| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lincoln, David G (9)
|
PVNCAP |
40.45 |
40.36 |
-0.09 |
| 2 |
Wall, Nicholas J (9)
|
PVOCCS |
41.25 |
40.50 |
-0.75 |
| 3 |
Erdas, Aaron (9)
|
PVNCAP |
41.43 |
40.88 |
-0.55 |
| 4 |
Foster, Aleksandr J (9)
|
PVOCCS |
41.15 |
42.59 |
1.44 |
| 5 |
Soundappan, Tristan M (9)
|
PVNCAP |
44.88 |
44.43 |
-0.45 |
| 6 |
Hergenroeder, Matthew L (9)
|
PVSTJS |
44.71 |
45.43 |
0.72 |
| 7 |
MacDonald, Edwin M (9)
|
PVOCCS |
50.13 |
47.33 |
-2.80 |
| 8 |
Brown, Lincoln N (9)
|
PVOCCS |
49.11 |
47.56 |
-1.55 |
| 9 |
Cabusao, Max I (9)
|
PVNCAP |
45.94 |
47.70 |
1.76 |
| 10 |
Morse, Brecken A (9)
|
PVSTJS |
|
48.19 |
- |
| 11 |
Diaz-Brignoni, Enzo N (9)
|
PVNCAP |
52.05 |
49.46 |
-2.59 |
| 12 |
Stephenson, Jordan C (9)
|
PVNCAP |
47.56 |
49.71 |
2.15 |
| 13 |
Samra, Nathan Y (9)
|
PVNCAP |
48.18 |
51.05 |
2.87 |
| 14 |
Woods, Evan M (9)
|
PVOCCS |
51.01 |
51.91 |
0.90 |
| 15 |
Robison, John B (9)
|
PVOCCS |
56.75 |
53.48 |
-3.27 |
| 16 |
Marra, David L (9)
|
PVSTJS |
51.73 |
54.96 |
3.23 |
| 17 |
Walter, Thomas E (9)
|
PVSTJS |
|
58.76 |
- |
| 18 |
Chiranjeevi, Ayush (9)
|
PVNCAP |
1:00.34 |
59.94 |
-0.40 |
| 19 |
Montgomery, Luke G (9)
|
PVSTJS |
|
1:00.40 |
- |
| 20 |
Grajkowski, Derrick L (9)
|
PVSTJS |
59.98 |
1:02.91 |
2.93 |
| 21 |
Moran, Jack E (9)
|
PVNCAP |
|
1:04.74 |
- |
| 22 |
Zhou, Jayden Y (9)
|
PVNCAP |
|
1:04.89 |
- |
| 23 |
McInnis, Michael C (9)
|
PVNCAP |
|
1:06.61 |
- |
| 24 |
Nichols, Ian F (9)
|
PVNCAP |
1:05.01 |
1:09.70 |
4.69 |
| 25 |
Tramel, Joseph A (9)
|
PVNCAP |
1:14.81 |
1:15.77 |
0.96 |
|
Baptiste, Isaac C (9)
|
PVPGPR |
|
DQ |
- |
|
Maier, William G (9)
|
PVMAKO |
42.62 |
NS |
- |
|
Murphy, Jack W (9)
|
PVSTJS |
|
NS |
- |