| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Delarosa, Joseph Christopher (12)
|
NTMAC |
30.97 |
29.05 |
-1.92 |
| 2 |
Seiders, Nathan C (12)
|
NTMARS |
30.68 |
29.96 |
-0.72 |
| 3 |
Nguyen, Avery N (11)
|
NTLAC |
30.68 |
30.35 |
-0.33 |
| 4 |
Wade, Liam B (12)
|
NTLAC |
31.56 |
30.59 |
-0.97 |
| 5 |
Clegg, Turner D (12)
|
NTLAC |
33.31 |
30.73 |
-2.58 |
| 6 |
Ali, Isley J (12)
|
NTMARS |
32.12 |
31.31 |
-0.81 |
| 7 |
Fancher, Landon C (12)
|
NTLAC |
31.51 |
31.32 |
-0.19 |
| 8 |
Avitia, Lukas C (12)
|
NTMAC |
32.52 |
31.41 |
-1.11 |
| 9 |
Moon, Andrew J (11)
|
NTMARS |
32.52 |
32.10 |
-0.42 |
| 10 |
Thomas, London X (11)
|
NTMTRO |
|
32.44 |
- |
| 11 |
Werth, Nolan M (11)
|
NTUN |
39.44 |
32.95 |
-6.49 |
| 12 |
Collins, Boaz Y (11)
|
NTMAC |
33.39 |
32.96 |
-0.43 |
| 13 |
Traub, Gabriel M (12)
|
NTLAC |
31.91 |
33.73 |
1.82 |
| 14 |
Hamer, Abi D (11)
|
NTMTRO |
|
34.27 |
- |
| 15 |
Sieg, Karl C (11)
|
NTLAC |
36.91 |
35.17 |
-1.74 |
| 16 |
Premmanee, Kuhnnachupb (11)
|
NTMAC |
34.47 |
35.26 |
0.79 |
| 17 |
Camargo Chavez, Antonio (12)
|
NTMTRO |
40.94 |
35.35 |
-5.59 |
| 18 |
Mares, Fabian (12)
|
NTMTRO |
|
35.47 |
- |
| 19 |
Sityar, Jacob L (11)
|
NTMAC |
35.65 |
36.07 |
0.42 |
| 20 |
Olsen, Christian E (11)
|
NTLAC |
34.74 |
36.88 |
2.14 |
| 21 |
Starnes, Drew M (12)
|
NTLAC |
35.90 |
37.71 |
1.81 |
| 22 |
Akula Kamalesh, Trinay (12)
|
NTLAC |
37.02 |
38.21 |
1.19 |
| 23 |
Tatari, Tyler A (11)
|
NTLAC |
37.25 |
38.30 |
1.05 |
| 24 |
Markle, Gavin R (11)
|
NTMTRO |
|
38.48 |
- |
| 25 |
Ramos, Jorge F (11)
|
NTMTRO |
47.33 |
40.22 |
-7.11 |
| 26 |
Camargo Chavez, Cesar (11)
|
NTMTRO |
46.24 |
40.32 |
-5.92 |
| 27 |
Willert, Matthew G (11)
|
NTMTRO |
40.52 |
40.34 |
-0.18 |
| 28 |
Hulme, Mitchell S (11)
|
NTMARS |
46.00 |
41.74 |
-4.26 |
| 29 |
Greiner, Mason M (12)
|
NTLAC |
46.76 |
42.63 |
-4.13 |
| 30 |
Tatari, Evan S (11)
|
NTLAC |
49.41 |
47.13 |
-2.28 |