| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hill, Kylee (10)
|
IABUR |
29.01 |
28.97 |
-0.04 |
| 2 |
Speers, Elizabeth (10)
|
IABUR |
30.78 |
30.54 |
-0.24 |
| 3 |
Morrison, Daphanie (10)
|
IAOTT |
31.02 |
30.65 |
-0.37 |
| 4 |
Lindeman, Olivia (10)
|
IACRSP |
32.14 |
31.12 |
-1.02 |
| 5 |
Roling, Rebecca (10)
|
IADUBQ |
31.84 |
31.27 |
-0.57 |
| 6 |
Klinkenborg, Madelyn M (10)
|
IABHCP |
31.70 |
31.56 |
-0.14 |
| 7 |
Miller, Kimberlee (10)
|
IANEWT |
31.99 |
31.68 |
-0.31 |
| 8 |
Baker, Kahlan (9)
|
IAMAQ |
33.46 |
31.72 |
-1.74 |
| 9 |
Engelkemier, Birdie J (10)
|
IAMUS |
31.80 |
32.07 |
0.27 |
| 10 |
Cloos, Elyse (10)
|
IADUBQ |
33.57 |
32.39 |
-1.18 |
| 11 |
Cook, Mallory (10)
|
IACCY |
33.26 |
32.64 |
-0.62 |
| 12 |
Folden, Claire E (10)
|
IADSMY |
33.86 |
33.52 |
-0.34 |
| 13 |
Hamner, Rowan E (10)
|
IASCFY |
33.88 |
34.41 |
0.53 |