| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Taylor, Jaime O (8)
|
MAWSY |
48.90 |
42.19 |
-6.71 |
| 2 |
Vlaeminck, Julian P (8)
|
MAEMAC |
45.29 |
45.42 |
0.13 |
| 3 |
Behun, William G (8)
|
MAUN-5 |
50.83 |
48.75 |
-2.08 |
| 4 |
Grimley, Jack P (8)
|
MARAD |
|
52.02 |
- |
| 5 |
Ritchey, Oliver N (7)
|
MAEMAC |
|
53.32 |
- |
| 6 |
Wanner, Aidan L (8)
|
MANHAC |
|
55.52 |
- |
| 7 |
Cessna, Luke C (8)
|
MANLAC |
54.88 |
55.69 |
0.81 |
| 8 |
Wiley, Nathaniel (8)
|
MAWASC |
|
56.26 |
- |
| 9 |
King, Bryce G (7)
|
MAEMAC |
|
58.15 |
- |
| 10 |
Kourt, Roman X (8)
|
MANHAC |
|
59.33 |
- |
| 11 |
Berezin, Stephan I (8)
|
MANLAC |
1:06.41 |
1:05.84 |
-0.57 |
| 12 |
Bodwell, Colton J (8)
|
MAWASC |
1:09.06 |
1:09.85 |
0.79 |
|
Caramenico, Matthew R (6)
|
MANHAC |
|
DQ |
- |
|
Simes, Cooper A (6)
|
MANHAC |
|
DQ |
- |
|
Small, Sebastian (8)
|
MASPFD |
|
DQ |
- |